Effect of suppressive DNA on CpG-induced immune activation

被引：90

作者：

Yamada, H ^{[1
]}

Gursel, I ^{[1
]}

Takeshita, F ^{[1
]}

Conover, J ^{[1
]}

Ishii, KJ ^{[1
]}

Gursel, M ^{[1
]}

Takeshita, S ^{[1
]}

Klinman, DM ^{[1
]}

机构：

[1] US FDA, Ctr Biol Evaluat & Res, Sect Retroviral Immunol, Bethesda, MD 20892 USA

来源：

JOURNAL OF IMMUNOLOGY | 2002年 / 169卷 / 10期

关键词：

D O I：

10.4049/jimmunol.169.10.5590

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Bacterial DNA and synthetic oligodeoxynucleotides (ODN) containing unmethylated CpG motifs stimulate a strong innate immune response. This stimulation can be abrogated by either removing the CpG DNA or adding inhibitory/suppressive motifs. Suppression is dominant over stimulation and is specific for CpG-induced immune responses (having no effect on LPS- or Con A-induced activation). Individual cells noncompetitively internalize both stimulatory and suppressive ODN. Studies using ODN composed of both stimulatory and suppressive motifs indicate that sequence recognition proceeds in a 5'-->3' direction, and that a 5' motif can block recognition of immediately 3' sequences. These findings contribute to our understanding of the immunomodulatory activity of DNA-based products and the rules that govern immune recognition of stimulatory and suppressive motifs.

引用

页码：5590 / 5594

页数：5

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