Endothelial cell migration on surfaces modified with immobilized adhesive peptides

被引:107
作者
Kouvroukoglou, S
Dee, KC
Bizios, R
McIntire, LV
Zygourakis, K
机构
[1] Rice Univ, Dept Chem Engn, Houston, TX 77251 USA
[2] Tulane Univ, Dept Biomed Engn, New Orleans, LA 70118 USA
[3] Rensselaer Polytech Inst, Dept Biomed Engn, Troy, NY 12180 USA
[4] Rice Univ, Dept Bioengn, Houston, TX 77251 USA
基金
美国国家科学基金会;
关键词
endothelial cell migration; persistent random walk; surface modification; adhesive peptides; synthetic vascular grafts;
D O I
10.1016/S0142-9612(99)00205-7
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Endothelial cell (EC) migration has been studied on aminophase surfaces with covalently bound RGDS and YIGSRG cell adhesion peptides. The fluorescent marker dansyl chloride was used to quantify the spatial distribution of the peptides on the modified surfaces. Peptides appeared to be distributed in uniformly dispersed large clusters separated by areas of lower peptide concentrations. We employed digital time-lapse video microscopy and image analysis to monitor EC migration on the modified surfaces and to reconstruct the cell trajectories. The persistent random walk model was then applied to analyze the cell displacement data and compute the mean root square speed, the persistence time, and the random motility coefficient of EC. We also calculated the time-averaged speed of cell locomotion. No differences in the speed of cell locomotion on the various substrates were noted. Immobilization of the cell adhesion peptides (RGDS and YIGSRG), however, significantly increased the persistence of cell movement and, thus, the random motility coefficient. These results suggest that immobilization of cell adhesion peptides on the surface of implantable biomaterials may lead to enhanced endothelization rates. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
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页码:1725 / 1733
页数:9
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