Fast high-performance liquid chromatography method for quality control of soy extracts

被引：30

作者：

Apers, S ^{[1
]}

Naessens, T ^{[1
]}

Van Den Steen, K ^{[1
]}

Cuyckens, F ^{[1
]}

Claeys, M ^{[1
]}

Pieters, L ^{[1
]}

Vlietinck, A ^{[1
]}

机构：

[1] Univ Instelling Antwerp, Dept Pharmaceut Sci, B-2610 Antwerp, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1038卷 / 1-2期

关键词：

Glycine max; food analysis; isoflavones; aglycones; glycosides;

D O I：

10.1016/j.chroma.2004.03.033

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Soy extracts contain a mixture of isoflavones belonging to the group of phytoestrogens. In the quality control of soy the amount of isoflavones, both aglycones and glycosides, is usually determined by means of reversed-phase HPLC-UV. On conventional C-18-material columns, long analysis times are required in order to separate this complex mixture. In order to speed up analysis, the separation was optimized using two linked monolithic silica-based reversed-phase C-18 columns. A spectacular decrease of the analysis time, i.e. almost three-fold, was achieved by applying a flow rate of 3-4 ml/min without loosing any separation efficiency. This analysis method for determination of isoflavones in soy extracts in less than 25 min was fully validated according to the ICH guidelines. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：107 / 112

页数：6

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