The recombinant 120-kilodalton protein of Ehrlichia chaffeensis, a potential diagnostic tool

被引:40
作者
Yu, XJ
CrocquetValdes, P
Cullman, LC
Walker, DH
机构
[1] UNIV TEXAS,MED BRANCH,DEPT PATHOL,GALVESTON,TX 77555
[2] MRL DIAGNOST,CYPRESS,CA 90630
关键词
D O I
10.1128/JCM.34.11.2853-2855.1996
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
DNA encoding two repeat units of the 120-kDa protein of Ehrlichia chaffeensis was cloned into the expression vector pGEX and expressed in Escherichia coli. The sensitivity and specificity of a dot blot assay for detection of human antibodies with the recombinant protein were 86 and 100%, respectively, compared with an indirect immunofluorescence assay.
引用
收藏
页码:2853 / 2855
页数:3
相关论文
共 10 条
[1]   EHRLICHIA-CHAFFEENSIS, A NEW SPECIES ASSOCIATED WITH HUMAN EHRLICHIOSIS [J].
ANDERSON, BE ;
DAWSON, JE ;
JONES, DC ;
WILSON, KH .
JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (12) :2838-2842
[2]   IDENTIFICATION OF THE ANTIGENIC CONSTITUENTS OF EHRLICHIA-CHAFFEENSIS [J].
CHEN, SM ;
DUMLER, JS ;
FENG, HM ;
WALKER, DH .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1994, 50 (01) :52-58
[3]   DIAGNOSIS OF HUMAN EHRLICHIOSIS WITH THE INDIRECT FLUORESCENT-ANTIBODY TEST - KINETICS AND SPECIFICITY [J].
DAWSON, JE ;
FISHBEIN, DB ;
ENG, TR ;
REDUS, MA ;
GREENE, NR .
JOURNAL OF INFECTIOUS DISEASES, 1990, 162 (01) :91-95
[4]   SEROLOGIC DIAGNOSIS OF HUMAN EHRLICHIOSIS USING 2 EHRLICHIA-CANIS ISOLATES [J].
DAWSON, JE ;
RIKIHISA, Y ;
EWING, SA ;
FISHBEIN, DB .
JOURNAL OF INFECTIOUS DISEASES, 1991, 163 (03) :564-567
[5]   ISOLATION AND CHARACTERIZATION OF AN EHRLICHIA SP FROM A PATIENT DIAGNOSED WITH HUMAN EHRLICHIOSIS [J].
DAWSON, JE ;
ANDERSON, BE ;
FISHBEIN, DB ;
SANCHEZ, JL ;
GOLDSMITH, CS ;
WILSON, KH ;
DUNTLEY, CW .
JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (12) :2741-2745
[6]   The interface between research and the diagnosis of an emerging tick-borne disease, human ehrlichiosis due to Ehrlichia chaffeensis [J].
Dawson, JE ;
Warner, CK ;
Standaert, S ;
Olson, JG .
ARCHIVES OF INTERNAL MEDICINE, 1996, 156 (02) :137-142
[7]   HUMAN EHRLICHIOSIS IN THE UNITED-STATES, 1985 TO 1990 [J].
FISHBEIN, DB ;
DAWSON, JE ;
ROBINSON, LE .
ANNALS OF INTERNAL MEDICINE, 1994, 120 (09) :736-743
[8]   UNEXPLAINED FEBRILE ILLNESSES AFTER EXPOSURE TO TICKS - INFECTION WITH AN EHRLICHIA [J].
FISHBEIN, DB ;
SAWYER, LA ;
HOLLAND, CJ ;
HAYES, EB ;
OKOROANYANWU, W ;
WILLIAMS, D ;
SIKES, RK ;
RISTIC, M ;
MCDADE, JE .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1987, 257 (22) :3100-3104
[9]   HUMAN INFECTION WITH EHRLICHIA-CANIS, A LEUKOCYTIC RICKETTSIA [J].
MAEDA, K ;
MARKOWITZ, N ;
HAWLEY, RC ;
RISTIC, M ;
COX, D ;
MCDADE, JE .
NEW ENGLAND JOURNAL OF MEDICINE, 1987, 316 (14) :853-856
[10]  
YU XJ, IN PRESS GENE