New cyclization mechanism for squalene: a ring-expansion step for the five-membered C-ring intermediate in hopene biosynthesis

被引：45

作者：

Hoshino, T ^{[1
]}

Kouda, M

Abe, T

Ohashi, S

机构：

[1] Niigata Univ, Fac Agr, Dept Appl Biol Chem, Niigata 9502181, Japan

[2] Niigata Univ, Grad Sch Sci & Technol, Niigata 9502181, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1999年 / 63卷 / 11期

关键词：

squalene; hopene; triterpene cyclase; 2,3-oxidosqualene;

D O I：

10.1271/bbb.63.2038

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three triterpenes having the 6/6/5-fused tri- and 6/6/6/5-fused tetracyclic skeletons were isolated from an incubation mixture of the mutated F601A enzyme, these products being in accordance with a Markovnikov closure. successful trapping of the tricyclic cationic intermediate by using the squalene analog having a highly nucleophilic hydroxyl group leads us to propose that the ring expansion process of the 5-membered C-ring is involved in the squalene cyclization cascade.

引用

页码：2038 / 2041

页数：4

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