An AP2 domain transcription factor HvCBF1 activates expression of cold-responsive genes in barley through interaction with a (G/a)(C/t)CGAC motif

被引:59
作者
Xue, GP [1 ]
机构
[1] CSIRO, Plant Ind, Indooroopilly, Qld 4068, Australia
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2002年 / 1577卷 / 01期
关键词
AP2; domain; transcriptional activator; ABA-independent; cold; barley; CRT/DRE;
D O I
10.1016/S0167-4781(02)00410-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A HvCBF1 cDNA encoding an AP2 domain-containing protein was isolated from barley leaves. Expression of HvCBF1 was induced in barley leaves by exposure to a low temperature (2 degreesC), but not by drought or abscisic acid (ABA) treatment. Functional analysis showed that HnuCBF1 was a transcriptional activator, capable of activating expression of reporter genes driven by a cold- and drought-responsive HVA1s promoter in barley leaves. Transactivation analysis of HvCBF1 on a number of synthetic oligonucleotides containing potential C-repeat (CRT)/dehydration-responsive element (DRE) derived from cold-responsive barley genes revealed that HvCBF1 interacted much more efficiently with a GCCGAC motif than a previously identified barley low-temperature-responsive element (LTRE), CCGAAA. A detailed base-substitution mutagenesis study revealed that only the CGAC sequence of the GCCGAC motif was highly conserved for interacting with HvCBF1. The promoter activity of the mutant motifs, ACCGAC and GTCGAC, was 36% and 75% of that of the GCCGAC motif, respectively. The base composition surrounding the GCCGAC motif also had a significant effect on the efficiency of the motif. These data suggest that barley HvCBF1 protein interacts with the (G/a)(C/t)CGAC motif and is involved in regulation of cold-responsive genes in barley via an ABA-independent signal transduction pathway. Crown Copyright (C) 2002 Published by Elsevier Science B.V All rights reserved.
引用
收藏
页码:63 / 72
页数:10
相关论文
共 35 条
[1]   THE 5'-REGION OF ARABIDOPSIS-THALIANA COR15A HAS CIS-ACTING ELEMENTS THAT CONFER COLD-REGULATED, DROUGHT-REGULATED AND ABA-REGULATED GENE-EXPRESSION [J].
BAKER, SS ;
WILHELM, KS ;
THOMASHOW, MF .
PLANT MOLECULAR BIOLOGY, 1994, 24 (05) :701-713
[2]   The role of ABA in freezing tolerance and cold acclimation in barley [J].
Bravo, LA ;
Zúñiga, GE ;
Alberdi, M ;
Corcuera, LJ .
PHYSIOLOGIA PLANTARUM, 1998, 103 (01) :17-23
[3]   MOLECULAR-CLONING AND CHARACTERIZATION OF COLD-REGULATED GENES IN BARLEY [J].
CATTIVELLI, L ;
BARTELS, D .
PLANT PHYSIOLOGY, 1990, 93 (04) :1504-1510
[4]   The barley (Hordeum vulgare L.) dehydrin multigene family:: sequences, allele types, chromosome assignments, and expression characteristics of 11 Dhn genes of cv Dicktoo [J].
Choi, DW ;
Zhu, B ;
Close, TJ .
THEORETICAL AND APPLIED GENETICS, 1999, 98 (08) :1234-1247
[5]   ACCUMULATION AND CHARACTERIZATION OF THE 75 KDA PROTEIN-INDUCED BY LOW-TEMPERATURE IN BARLEY [J].
CROSATTI, C ;
RIZZA, F ;
CATTIVELLI, L .
PLANT SCIENCE, 1994, 97 (01) :39-46
[6]   LOW-TEMPERATURE-RESPONSIVE BARLEY GENES HAVE DIFFERENT CONTROL MECHANISMS [J].
DUNN, MA ;
GODDARD, NJ ;
ZHANG, L ;
PEARCE, RS ;
HUGHES, MA .
PLANT MOLECULAR BIOLOGY, 1994, 24 (06) :879-888
[7]   Identification of promoter elements in a low-temperature-responsive gene (blt4.9) from barley (Hordeum vulgare L.) [J].
Dunn, MA ;
White, AJ ;
Vural, S ;
Hughes, MA .
PLANT MOLECULAR BIOLOGY, 1998, 38 (04) :551-564
[8]   Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression [J].
Gilmour, SJ ;
Zarka, DG ;
Stockinger, EJ ;
Salazar, MP ;
Houghton, JM ;
Thomashow, MF .
PLANT JOURNAL, 1998, 16 (04) :433-442
[9]   CHARACTERIZATION OF 2 BARLEY GENES THAT RESPOND RAPIDLY TO DEHYDRATION STRESS [J].
GROSSI, M ;
GULLI, M ;
STANCA, AM ;
CATTIVELLI, L .
PLANT SCIENCE, 1995, 105 (01) :71-80
[10]   DEVELOPMENTAL AND ORGAN-SPECIFIC EXPRESSION OF AN ABA-INDUCED AND STRESS-INDUCED PROTEIN IN BARLEY [J].
HONG, BM ;
BARG, R ;
HO, THD .
PLANT MOLECULAR BIOLOGY, 1992, 18 (04) :663-674