Electrochemical stripping detection of DNA hybridization based on cadmium sulfide nanoparticle tags

被引：132

作者：

Wang, J ^{[1
]}

Liu, GD ^{[1
]}

Polsky, R ^{[1
]}

Merkoçi, A ^{[1
]}

机构：

[1] New Mexico State Univ, Dept Chem & Biochem, Las Cruces, NM 88003 USA

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2002年 / 4卷 / 09期

基金：

美国国家科学基金会;

关键词：

nanoparticles; DNA hybridization; cadmium sulfide; stripping potentiometry;

D O I：

10.1016/S1388-2481(02)00434-4

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

We report on the detection of DNA hybridization in connection to cadmium sulfide nanoparticle tracers and electrochemical stripping measurements of the cadmium. A nanoparticle-promoted cadmium precipitation is used to enlarge the nanoparticle tag and amplify the stripping DNA hybridization signal. In addition to measurements of the dissolved cadmium ion c demonstrate solid-state measurements following a 'magnetic' collection of the magnetic-bead/DNA-hybrid/CdS-tracer assembly onto a thick-film electrode transducer. The new protocol combines the amplification features of nanoparticle/polynucleotides assemblies and highly sensitive stripping potentiometric detection of cadmium. with an effective magnetic isolation of the duplex. The low detection limit (100 fmol) is coupled to good reproducibility (RSD = 6%). Prospects for using binary inorganic colloids for multi-target detection are discussed. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：722 / 726

页数：5

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