Six new microsatellite markers for the toxic marine dinoflagellate Alexandrium tamarense

被引：16

作者：

Alpermann, Tilman J.

John, Uwe

Medlin, Linda K.

Edwards, Keith J.

Hayes, Paul K.

Evans, Katharine M.

机构：

[1] Alfred Wegener Inst Polar & Marine Res, D-27570 Bremerhaven, Germany

[2] Univ Bristol, Sch Biol Sci, Bristol BS8 1UG, Avon, England

来源：

MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 04期

基金：

英国自然环境研究理事会;

关键词：

Alexandrium tamarense 'species complex'; dinoflagellate; harmful algal blooms; microsatellites;

D O I：

10.1111/j.1471-8286.2006.01432.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the characterization of six new microsatellite loci for the toxic marine dinoflagellate Alexandrium tamarense (North American ribotype), using 56 isolates from a range of locations. The numbers of alleles per locus ranged from five to nine and gene diversities ranged from 0.041 to 0.722. We tested primers for these six loci on other A. tamarense ribotypes and on other Alexandrium species; the results suggest that the primers are specific to A. tamarense isolates belonging to the North American ribotype.

引用

页码：1057 / 1059

页数：3

共 11 条

[1]

[Anonymous], 2000, ARLEQUIN SOFTWARE PO

[2]

Balech E., 1995, The genus Alexandrium Halim (Dinoflagellata)

[3]

Doyle JJ., 1987, FOCUS, V19, P11, DOI DOI 10.2307/2419362

[4]

Edwards KJ, 1996, BIOTECHNIQUES, V20, P758

[5] The application of a molecular clock based on molecular sequences and the fossil record to explain biogeographic distributions within the Alexandrium tamarense "species complex" (Dinophyceae) [J].

John, U ;

Fensome, RA ;

Medlin, LK .

MOLECULAR BIOLOGY AND EVOLUTION, 2003, 20 (07) :1015-1027

[6] Development of microsatellite markers in the toxic dinoflagellate Alexandrium tamarense (Dinophyceae) [J].

Nagai, S ;

Lian, C ;

Hamaguchi, M ;

Matsuyama, Y ;

Itakura, S ;

Hogetsu, T .

MOLECULAR ECOLOGY NOTES, 2004, 4 (01) :83-85

[7]

Net M., 1987, MOL EVOLUTIONARY GEN

[8]

ROTTGERS S, 2002, THESIS J LIEBIG U GI

[9]

Rozen S, 2000, Methods Mol Biol, V132, P365

[10] IDENTIFICATION OF GROUP-SPECIFIC AND STRAIN-SPECIFIC GENETIC-MARKERS FOR GLOBALLY DISTRIBUTED ALEXANDRIUM (DINOPHYCEAE) .2. SEQUENCE-ANALYSIS OF A FRAGMENT OF THE LSU RIBOSOMAL-RNA GENE [J].

SCHOLIN, CA ;

HERZOG, M ;

SOGIN, M ;

ANDERSON, DM .

JOURNAL OF PHYCOLOGY, 1994, 30 (06) :999-1011

← 1 2 →