RETRACTED: A medium for the presumptive detection of Enterobacter sakazakii in infant formula (Retracted Article. See vol 23, pg 409, 2006)

被引:21
作者
Leuschner, RGK [1 ]
Baird, F [1 ]
Donald, B [1 ]
Cox, LJ [1 ]
机构
[1] Cent Sci Lab, Dept Environm Food & Rural Affairs, York YO41 1LZ, N Yorkshire, England
关键词
Enterobacter sakazakii; selective medium; detection; infant formula;
D O I
10.1016/j.fm.2003.12.002
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A differential-elective medium for the isolation of Enterobacter sakazakii was developed. It is based on the presence of an enzyme alpha-glucosidase in this species that can differentiate this organism from other members of the family Enterobacteriaceae. Nutrient agar (NA) was supplemented with 4-methyl-umbelliferyl alpha-D-glucoside (alpha-MUG). This Substrate was metabolized by the enzyme to yield an UV fluorescent product. E sakakakii formed yellow pigmented UV fluorescent colonies on NA with alpha-MUG. Fifty-six E. sakazakii strains from culture collections were examined and found positive for both characteristics. Fifty-eight formula products from 11 countries were sampled in their entity by using a pre- and selective enrichment method following ISO 8523. The selective enrichment was streaked on NA + alpha-MUG and violet red brilliant green (VRBG) agar. Twenty-one (36%) of products were positive for Enterobacteriaceae. E. sakazakii was isolated from eight out of 58 infant formula products representing 13.8%. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:527 / 533
页数:7
相关论文
共 26 条
  • [1] ALDOVA E, 1983, ZBL BAKT-INT J MED M, V256, P103
  • [2] Enterobacter sakazakii infection in the newborn
    Bar-Oz, B
    Preminger, A
    Peleg, O
    Block, C
    Arad, I
    [J]. ACTA PAEDIATRICA, 2001, 90 (03) : 356 - 358
  • [3] 3 CASES OF NEONATAL MENINGITIS CAUSED BY ENTEROBACTER-SAKAZAKII IN POWDERED MILK
    BIERING, G
    KARLSSON, S
    CLARK, NC
    JONSDOTTIR, KE
    LUDVIGSSON, P
    STEINGRIMSSON, O
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (09) : 2054 - 2056
  • [4] Cluster of neonatal infections in Jerusalem due to unusual biochemical variant of Enterobacter sakazakii
    Block, C
    Peleg, O
    Minster, N
    Bar-Oz, B
    Simhon, A
    Arad, I
    Shapiro, M
    [J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2002, 21 (08) : 613 - 616
  • [5] Desiccation and heat tolerance of Enterobacter sakazakii
    Breeuwer, P
    Lardeau, A
    Peterz, M
    Joosten, HM
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2003, 95 (05) : 967 - 973
  • [6] EPIDEMIOLOGIC TYPING OF ENTEROBACTER-SAKAZAKII IN 2 NEONATAL NOSOCOMIAL OUTBREAKS
    CLARK, NC
    HILL, BC
    OHARA, CM
    STEINGRIMSSON, O
    COOKSEY, RC
    [J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 1990, 13 (06) : 467 - 472
  • [7] COX LJ, 1988, J APPL BACTERIOL, V65, pS237, DOI 10.1111/j.1365-2672.1988.tb04654.x
  • [8] BIOCHEMICAL-IDENTIFICATION OF NEW SPECIES AND BIOGROUPS OF ENTEROBACTERIACEAE ISOLATED FROM CLINICAL SPECIMENS
    FARMER, JJ
    DAVIS, BR
    HICKMANBRENNER, FW
    MCWHORTER, A
    HUNTLEYCARTER, GP
    ASBURY, MA
    RIDDLE, C
    WATHENGRADY, HG
    ELIAS, C
    FANNING, GR
    STEIGERWALT, AG
    OHARA, CM
    MORRIS, GK
    SMITH, PB
    BRENNER, DJ
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1985, 21 (01) : 46 - 76
  • [9] ENTEROBACTER-SAKAZAKII - A NEW SPECIES OF ENTEROBACTERIACEAE ISOLATED FROM CLINICAL SPECIMENS
    FARMER, JJ
    ASBURY, MA
    HICKMAN, FW
    BRENNER, DJ
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1980, 30 (03): : 569 - 584
  • [10] FARMER JJ, 1992, MANUAL CLIN MICROBIO, P360