The SH2D2A gene encoding the T-cell-specific adapter protein (TSAd) is localized centromeric to the CD1 gene cluster on human Chromosome 1

被引:13
作者
Dai, KZ
Vergnaud, G
Ando, A
Inoko, H
Spurkland, A [1 ]
机构
[1] Natl Hosp Norway, Inst Immunol, N-0027 Oslo, Norway
[2] Univ Paris 11, Inst Genet & Microbiol, F-91405 Orsay, France
[3] Tokai Univ, Sch Med, Dept Mol Life Sci, Kanagawa 2591193, Japan
关键词
adapter protein; SH2D2A gene; alternative splicing; single-nucleotide polymorphism; linkage analysis;
D O I
10.1007/s002510050029
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The SH2D2A gene encoding the T-cell-specific adapter protein (TSAd), was isolated from a human Chromosome (Chr) 1 cosmid library (LLNL, UK HGMP). The gene spans 11 kilobases and contains nine exons and eight introns. Four alternative transcript variants were observed in activated T cells. Three single-nucleotide polymorphisms were identified within intron 2. A variable number of GA repeats was found at position -340 from the first coding ATG. Linkage analysis using this marker in eight CEPH families showed that the SH2D2A gene is located close to the D1S2624 marker on Chr 1q21-1q22. Physical mapping of a PAC and BAC contig containing the CD1 gene cluster telomeric to D1S2624 failed to identify a clone harboring the SH2D2A gene. Thus the SH2D2A gene is located centromeric to the CD1 gene cluster on Chr 1.
引用
收藏
页码:179 / 185
页数:7
相关论文
共 23 条
[1]   Regulation of the plasminogen activator inhibitor type-2 gene in monocytes: Localization of an upstream transcriptional silencer [J].
Antalis, TM ;
Costelloe, E ;
Muddiman, J ;
Ogbourne, S ;
Donnan, K .
BLOOD, 1996, 88 (10) :3686-3697
[2]   Molecular genetics of coagulation factor VIII gene and haemophilia A [J].
Antonarakis, SE .
HAEMOPHILIA, 1998, 4 :1-11
[3]  
Ausubel FM., 1994, Curr. Protoc. Mol. Biol
[4]   SH2 and SH3-containing adaptor proteins: Redundant or Independent mediators of intracellular signal transduction [J].
Birge, RB ;
Knudsen, BS ;
Besser, D ;
Hanafusa, H .
GENES TO CELLS, 1996, 1 (07) :595-613
[5]  
Chen YY, 1996, J BIOL CHEM, V271, P5183
[6]  
CRABTREE GR, 1994, ANNU REV BIOCHEM, V63, P1045, DOI 10.1146/annurev.bi.63.070194.005145
[7]   A comprehensive genetic map of the human genome based on 5,264 microsatellites [J].
Dib, C ;
Faure, S ;
Fizames, C ;
Samson, D ;
Drouot, N ;
Vignal, A ;
Millasseau, P ;
Marc, S ;
Hazan, J ;
Seboun, E ;
Lathrop, M ;
Gyapay, G ;
Morissette, J ;
Weissenbach, J .
NATURE, 1996, 380 (6570) :152-154
[8]   RECOGNITION OF A HIGH-AFFINITY PHOSPHOTYROSYL PEPTIDE BY THE SRC HOMOLOGY-2 DOMAIN OF P56(LCK) [J].
ECK, MJ ;
SHOELSON, SE ;
HARRISON, SC .
NATURE, 1993, 362 (6415) :87-91
[9]   Evolutionary significance of intra-genome duplications on human chromosomes [J].
Endo, T ;
Imanishi, T ;
Gojobori, T ;
Inoko, H .
GENE, 1997, 205 (1-2) :19-27
[10]   Databases on transcriptional regulation: TRANSFAC, TRRD and COMPEL [J].
Heinemeyer, T ;
Wingender, E ;
Reuter, I ;
Hermjakob, H ;
Kel, AE ;
Kel, OV ;
Ignatieva, EV ;
Ananko, EA ;
Podkolodnaya, OA ;
Kolpakov, FA ;
Podkolodny, NL ;
Kolchanov, NA .
NUCLEIC ACIDS RESEARCH, 1998, 26 (01) :362-367