A simple 96-well microplate method for estimation of total polyphenol content in seaweeds

被引：366

作者：

Zhang, Qing ^{[1
]}

Zhang, Junzeng ^{[1
]}

Shen, Jingkai ^{[1
]}

Silva, Angelica ^{[1
]}

Dennis, Dorothy A. ^{[1
]}

Barrow, Colin J. ^{[1
]}

机构：

[1] Ocean Nutr Canada Ltd, Dartmouth, NS B2Y 4T6, Canada

来源：

JOURNAL OF APPLIED PHYCOLOGY | 2006年 / 18卷 / 3-5期

基金：

加拿大自然科学与工程研究理事会;

关键词：

seaweed; marine algae; total polyphenol; estimation; 96-well microplate;

D O I：

10.1007/s10811-006-9048-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Seaweed polyphenols are potent antioxidants and have also been shown to have alpha-glucosidase inhibiting activity. In our continuous efforts to develop new marine-based nutraceuticals and functional food ingredients, we have investigated many algal species collected on the Atlantic coast of Canada. A simple method for estimating the total polyphenol content in seaweeds and their extracts was developed based on the classic Folin-Ciocalteau colorimetric reaction. By using the 96-well microplate and a microplate reader, this new method saves experimental time, significantly reduces the amount of sample required, handles large number of samples in one experiment, and also improves the repeatability of the results. A number of algal samples collected on the seashore of Nova Scotia, Canada, were analyzed for their levels of polyphenol content using this microplate-based method. The antioxidant activity of these samples was also assessed by using DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging assay. The results showed that there is a strong correlation between the total polyphenol content and the potency of antioxidant effect.

引用

页码：445 / 450

页数：6

共 28 条

[1] Inhibition of HIV-1 reverse transcriptase and protease by phlorotannins from the brown alga Ecklonia cava [J].