The design of dehydrogenase enzymes for use in a biofuel cell: the role of genetically introduced peptide tags in enzyme immobilization on electrodes

被引：26

作者：

Halliwell, CM

Simon, E

Toh, CS

Cass, AEG ^{[1
]}

Bartlett, PN

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Biochem, London SW7 2AY, England

[2] Univ Southampton, Dept Chem, Southampton SO15 5RR, Hants, England

来源：

BIOELECTROCHEMISTRY | 2002年 / 55卷 / 1-2期

关键词：

enzyme immobilization; bioanode; biofuel cell; cofactor recycling; NAD(+);

D O I：

10.1016/S1567-5394(01)00172-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The immobilization of the mutants of L-lactate dehydrogenase (LDH) on poly(aniline) (PANi) composite films has been investigated. Mutants possessing peptide tags of varying charge and nucleophilicity were created to probe the nature of the interaction between the protein and PANi. These results are significant for the development of a 'generic' approach to the immobilization of enzymes and other proteins. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：21 / 23

页数：3

相关论文

共 11 条

[1] CLONING, EXPRESSION AND COMPLETE NUCLEOTIDE-SEQUENCE OF THE BACILLUS-STEAROTHERMOPHILUS L-LACTATE DEHYDROGENASE GENE [J].

BARSTOW, DA ;

CLARKE, AR ;

CHIA, WN ;

WIGLEY, D ;

SHARMAN, AF ;

HOLBROOK, JJ ;

ATKINSON, T ;

MINTON, NP .

GENE, 1986, 46 (01) :47-55

[2] The oxidation of β-nicotinamide adenine dinucleotide (NADH) at poly(aniline)-coated electrodes Part II.: Kinetics of reaction at poly(aniline)- poly(styrenesulfonate) composites [J].

Bartlett, PN ;

Wallace, ENK .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2000, 486 (01) :23-31

[3] Poly(aniline)-poly(acrylate) composite films as modified electrodes for the oxidation of NADH [J].

Bartlett, PN ;

Simon, E .

PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2000, 2 (11) :2599-2606

[4] Oxidation of beta-nicotinamide adenine dinucleotide (NADH) at poly(aniline)-coated electrodes [J].

Bartlett, PN ;

Birkin, PR ;

Wallace, ENK .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS, 1997, 93 (10) :1951-1960

[5] Introduction of a (poly)histidine tag in L-lactate dehydrogenase produces a mixture of active and inactive molecules [J].

Halliwell, CM ;

Morgan, G ;

Ou, CP ;

Cass, AEG .

ANALYTICAL BIOCHEMISTRY, 2001, 295 (02) :257-261

[6] Combination of electrochemistry with concurrent reduction and substitution chemistry to provide a facile and versatile tool for preparing highly functionalized polyanilines [J].

Han, CC ;

Hseih, WD ;

Yeh, JY ;

Hong, SP .

CHEMISTRY OF MATERIALS, 1999, 11 (02) :480-486

[7] Evaluation of genetically attached histidine affinity tails for purification of lactate dehydrogenase from transgenic tobacco [J].

Mejàre, M ;

Lilius, G ;

Bülow, L .

PLANT SCIENCE, 1998, 134 (01) :103-114

[8] A methanol/dioxygen biofuel cell that uses NAD+-dependent dehydrogenases as catalysts:: application of an electro-enzymatic method to regenerate nicotinamide adenine dinucleotide at low overpotentials [J].

Palmore, GTR ;

Bertschy, H ;

Bergens, SH ;

Whitesides, GM .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1998, 443 (01) :155-161

[9]

VOET D, 1995, BIOCHEMISTRY-US, P56

[10] STRUCTURE OF A TERNARY COMPLEX OF AN ALLOSTERIC LACTATE-DEHYDROGENASE FROM BACILLUS-STEAROTHERMOPHILUS AT 2.5 A RESOLUTION [J].

WIGLEY, DB ;

GAMBLIN, SJ ;

TURKENBURG, JP ;

DODSON, EJ ;

PIONTEK, K ;

MUIRHEAD, H ;

HOLBROOK, JJ .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 223 (01) :317-335