Calcium elevation-dependent and attenuated resting calcium-dependent abscisic acid induction of stomatal closure and abscisic acid-induced enhancement of calcium sensitivities of S-type anion and inward-rectifying K+ channels in Arabidopsis guard cells

被引:128
作者
Siegel, Robert S. [1 ,2 ]
Xue, Shaowu [1 ,2 ]
Murata, Yoshiyuki [1 ,2 ,3 ]
Yang, Yingzhen [1 ,2 ]
Nishimura, Noriyuki [1 ,2 ]
Wang, Angela [1 ,2 ]
Schroeder, Julian I. [1 ,2 ]
机构
[1] Univ Calif San Diego, Div Biol Sci, Cell & Dev Biol Sect, La Jolla, CA 92093 USA
[2] Univ Calif San Diego, Ctr Mol Genet, La Jolla, CA 92093 USA
[3] Okayama Univ, Dept Agr, Okayama 7008530, Japan
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
guard cell; Ca2+; intracellular Ca2+; abi1; signal transduction; CYTOSOLIC-FREE CALCIUM; PLASMA-MEMBRANE; SIGNAL-TRANSDUCTION; ION CHANNELS; VICIA-FABA; CYTOPLASMIC CALCIUM; GENE-EXPRESSION; FLUORESCENT INDICATORS; POTASSIUM CHANNELS; HYDROGEN-PEROXIDE;
D O I
10.1111/j.1365-313X.2009.03872.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
P>Stomatal closure in response to abscisic acid depends on mechanisms that are mediated by intracellular [Ca2+] ([Ca2+](i)), and also on mechanisms that are independent of [Ca2+](i) in guard cells. In this study, we addressed three important questions with respect to these two predicted pathways in Arabidopsis thaliana. (i) How large is the relative abscisic acid (ABA)-induced stomatal closure response in the [Ca2+](i)-elevation-independent pathway? (ii) How do ABA-insensitive mutants affect the [Ca2+](i)-elevation-independent pathway? (iii) Does ABA enhance (prime) the Ca2+ sensitivity of anion and inward-rectifying K+ channel regulation? We monitored stomatal responses to ABA while experimentally inhibiting [Ca2+](i) elevations and clamping [Ca2+](i) to resting levels. The absence of [Ca2+](i) elevations was confirmed by ratiometric [Ca2+](i) imaging experiments. ABA-induced stomatal closure in the absence of [Ca2+](i) elevations above the physiological resting [Ca2+](i) showed only approximately 30% of the normal stomatal closure response, and was greatly slowed compared to the response in the presence of [Ca2+](i) elevations. The ABA-insensitive mutants ost1-2, abi2-1 and gca2 showed partial stomatal closure responses that correlate with [Ca2+](i)-dependent ABA signaling. Interestingly, patch-clamp experiments showed that exposure of guard cells to ABA greatly enhances the ability of cytosolic Ca2+ to activate S-type anion channels and down-regulate inward-rectifying K+ channels, providing strong evidence for a Ca2+ sensitivity priming hypothesis. The present study demonstrates and quantifies an attenuated and slowed ABA response when [Ca2+](i) elevations are directly inhibited in guard cells. A minimal model is discussed, in which ABA enhances (primes) the [Ca2+](i) sensitivity of stomatal closure mechanisms.
引用
收藏
页码:207 / 220
页数:14
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