N-Glycolylneuraminic Acid as a Receptor for Influenza A Viruses

被引：76

作者：

Broszeit, Frederik ^{[1
]}

Tzarum, Netanel ^{[2
]}

Zhu, Xueyong ^{[2
]}

Nemanichvili, Nikoloz ^{[3
]}

Eggink, Dirk ^{[4
]}

Leenders, Tim ^{[1
]}

Li, Zeshi ^{[1
]}

Liu, Lin ^{[5
]}

Wolfert, Margreet A. ^{[1
,5
]}

Papanikolaou, Andreas ^{[3
]}

Martinez-Romero, Caries ^{[6
,7
]}

Gagarinov, Ivan A. ^{[1
]}

Yu, Wenli ^{[2
]}

Garcia-Sastre, Adolfo ^{[6
,7
,8
]}

Wennekes, Tom ^{[1
]}

Okamatsu, Masatoshi ^{[9
]}

Verheije, Monique H. ^{[3
]}

Wilson, Ian A. ^{[2
,10
]}

Boons, Geert-Jan ^{[1
,5
]}

de Vries, Robert P. ^{[1
]}

机构：

[1] Univ Utrecht, Utrecht Inst Pharmaceut Sci, Dept Chem Biol & Drug Discovery, NL-3584 CG Utrecht, Netherlands

[2] Scripps Res Inst, Dept Integrat Struct & Computat Biol, La Jolla, CA 92037 USA

[3] Univ Utrecht, Fac Vet Med, Dept Pathobiol, NL-3584 CL Utrecht, Netherlands

[4] Amsterdam Med Ctr, Dept Expt Virol, Amsterdam, Netherlands

[5] Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA

[6] Icahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA

[7] Icahn Sch Med Mt Sinai, Global Hlth & Emerging Pathogens Inst, New York, NY 10029 USA

[8] Icahn Sch Med Mt Sinai, Dept Med, Div Infect Dis, New York, NY 10029 USA

[9] Hokkaido Univ, Grad Sch Vet Med, Dept Dis Control, Lab Microbiol, Sapporo, Hokkaido, Japan

[10] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

CELL REPORTS | 2019年 / 27卷 / 11期

关键词：

SIALIC ACIDS; ENZYMATIC-ACTIVITY; SCALE PRODUCTION; SPECIFICITY; BINDING; HEMAGGLUTININ; NEURAMINIDASE; RECOGNITION; SIALYLGLYCOPEPTIDE; EXPRESSION;

D O I：

10.1016/j.celrep.2019.05.048

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

A species barrier for the influenza A virus is the differential expression of sialic acid, which can either be alpha 2,3-linked for avians or alpha 2,6-linked for human viruses. The influenza A virus hosts also express other species-specific sialic acid derivatives. One major modification at C-5 is N-glycolyl (NeuGc), instead of N-acetyl (NeuAc). N-glycolyl is mammalian specific and expressed in pigs and horses, but not in humans, ferrets, seals, or dogs. Hemagglutinin (HA) adaptation to either N-acetyl or N-glycolyl is analyzed on a sialoside microarray containing both alpha 2,3- and alpha 2,6-linkage modifications on biologically relevant N-glycans. Binding studies reveal that avian, human, and equine HAs bind either N-glycolyl or N-acetyl. Structural data on N-glycolyl binding HA proteins of both H5 and H7 origin describe this specificity. Neuraminidases can cleave N-glycolyl efficiently, and tissue-binding studies reveal strict species specificity. The exclusive manner in which influenza A viruses differentiate between N-glycolyl and N-acetyl is indicative of selection.

引用

页码：3284 / +

页数：17

共 69 条

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