Immunolocalization of PICK1 in the ascending auditory pathways of the adult rat

被引:6
作者
McInvale, AC
Staudinger, J
Harlan, RE
Garcia, MM
机构
[1] Tulane Univ, Sch Med, Program Neurosci, New Orleans, LA 70112 USA
[2] Univ Kansas, Dept Pharmacol Toxicol & Therapeut, Lawrence, KS 66045 USA
[3] Tulane Univ, Sch Med, Dept Struct & Cellular Biol, New Orleans, LA 70112 USA
[4] Tulane Univ, Sch Med, Dept Otolaryngol, New Orleans, LA 70112 USA
关键词
PDZ domains; PKC; AMPA receptors; postsynaptic density;
D O I
10.1002/cne.10328
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Protein that interacts with C-kinase a (PICKI) is a PDZ domain protein that interacts with many binding partners in the central nervous system (CNS), including activated protein kinase Calpha and subunits of the AMPA subtype of glutamate receptor. Almost nothing is known about the anatomic distribution of PICKI in the intact adult CNS. By using PICK1 antisera and peroxidase immunocytochemistry, we report on the distribution of PICK1 in the ascending pathways of the central auditory system of the adult rat. PICK1-immunoreactivity (ir) was observed in many component nuclei of the central auditory system, including the dorsal cochlear nucleus, anteroventral cochlear nucleus, posteroventral cochlear nucleus, some divisions of the superior olivary complex, inferior colliculus, medial geniculate body, and primary auditory cortex. The general staining pattern for PICK1-immunoreactivity was somatodendritic with scattered puncta in neuropil and somatodendritic regions. The distribution of PICK1 partially overlaps with PKCalpha and glutamate receptor subunits such as GluR2. These data suggest that PICK1 may function in the regulation of PKCalpha and GluR2 localization in components of the rat auditory system, which may be a fundamental mechanism of synaptic transmission and/or plasticity.
引用
收藏
页码:382 / 394
页数:13
相关论文
共 71 条
  • [1] BIOTIN AMPLIFICATION OF BIOTIN AND HORSERADISH-PEROXIDASE SIGNALS IN HISTOCHEMICAL STAINS
    ADAMS, JC
    [J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1992, 40 (10) : 1457 - 1463
  • [2] Cell signalling: MAGUK magic
    Anderson, JM
    [J]. CURRENT BIOLOGY, 1996, 6 (04) : 382 - 384
  • [3] [Anonymous], 1999, Chemoarchitectonic atlas of the rat forebrain
  • [4] Regulation of AMPA receptor endocytosis by a signaling mechanism shared with LTD
    Beattie, EC
    Carroll, RC
    Yu, X
    Morishita, W
    Yasuda, H
    von Zastrow, M
    Malenka, RC
    [J]. NATURE NEUROSCIENCE, 2000, 3 (12) : 1291 - 1300
  • [5] Protein targeting and calcium signaling microdomains in neuronal cells
    Blackstone, C
    Sheng, M
    [J]. CELL CALCIUM, 1999, 26 (05) : 181 - 192
  • [6] Presynaptic clustering of mGluR7a requires the PICK1PDZ domain binding site
    Boudin, H
    Doan, A
    Xia, J
    Shigemoto, R
    Huganir, RL
    Worley, P
    Craig, AM
    [J]. NEURON, 2000, 28 (02) : 485 - 497
  • [7] Molecular determinants for PICK1 synaptic aggregation and mGluR7a receptor coclustering
    Boudin, H
    Craig, AM
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (32) : 30270 - 30276
  • [8] Interactions between AMPA receptors and intracellular proteins
    Braithwaite, SP
    Meyer, G
    Henley, JM
    [J]. NEUROPHARMACOLOGY, 2000, 39 (06) : 919 - 930
  • [9] EphrinB ligands recruit GRIP family PDZ adaptor proteins into raft membrane microdomains
    Brückner, K
    Labrador, JP
    Scheiffele, P
    Herb, A
    Seeburg, PH
    Klein, R
    [J]. NEURON, 1999, 22 (03) : 511 - 524
  • [10] Burette A, 1999, J COMP NEUROL, V411, P601