CC chemokine ligand 2 down-modulation by selected Toll-like receptor agonist combinations contributes to T helper 1 polarization in human dendritic cells

被引：23

作者：

Del Corno, Manuela ^{[1
]}

Michienzi, Alessandro ^{[1
]}

Masotti, Andrea ^{[2
]}

Da Sacco, Letizia ^{[2
]}

Bottazzo, Gian Franco ^{[2
]}

Belardelli, Filippo ^{[1
]}

Gessani, Sandra ^{[1
]}

机构：

[1] Ist Super Sanita, Dept Cell Biol & Neurosci, I-00161 Rome, Italy

[2] Childrens Hosp Bambino Gesu, Lab Gene Express Microarrays, Rome, Italy

来源：

BLOOD | 2009年 / 114卷 / 04期

关键词：

MONOCYTE CHEMOATTRACTANT PROTEIN-1; NF-KAPPA-B; PERIPHERAL-BLOOD MONOCYTES; GRANULOMA-FORMATION; BETA-CHEMOKINES; IMMUNE-SYSTEM; HIV-1; GP120; IFN-BETA; EXPRESSION; INDUCTION;

D O I：

10.1182/blood-2009-01-199406

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

Toll-like receptor (TLR) signaling activation by pathogens is critical to the induction of immune responses, and demands tight regulation. We describe in this study that CC chemokine ligand 2 (CCL2) secretion triggered by TLR4 or TLR8 engagement is strongly inhibited upon simultaneous activation of both TLRs in human monocyte-derived dendritic cells (DCs). Impaired CCL2 secretion occurs concomitantly to interleukin-12 up-regulation, being part of a complex regulatory circuit ensuring optimal T helper type 1 polarization. Interestingly, triggering selected TLRs or their combinations differently affects nuclear factor-kappa B p65 activation and microRNA expression. Overall, these results indicate that CCL2 supplies an important immunomodulatory role to DCs, and may contribute to dictate the cytokine profile in T helper type 1 responses induced by DCs. (Blood. 2009; 114: 796-806)

引用

页码：796 / 806

页数：11

共 52 条

[1]

Myeloid differentiation factor 88-dependent and -independent pathways in Toll-like receptor signaling [J].

Akira, S ;

Hoshino, K .

JOURNAL OF INFECTIOUS DISEASES, 2003, 187 :S356-S363

[2]

MyD88-dependent and MyD88-independent pathways in synergy, priming, and tolerance between TLR agonists [J].

Bagchi, Aranya ;

Herrup, Elizabeth A. ;

Warren, H. Shaw ;

Trigilio, James ;

Shin, Hae-Sook ;

Valentine, Catherine ;

Hellman, Judith .

JOURNAL OF IMMUNOLOGY, 2007, 178 (02) :1164-1171

[3]

MicroRNAs: new regulators of immune cell development and function [J].

Baltimore, David ;

Boldin, Mark P. ;

O'Connell, Ryan M. ;

Rao, Dinesh S. ;

Taganov, Konstantin D. .

NATURE IMMUNOLOGY, 2008, 9 (08) :839-845

[4]

Controllers of the immune system and a new promise for immunotherapy [J].

Banchereau, J ;

Paczesny, S ;

Blanco, P ;

Bennett, L ;

Pascual, V ;

Fay, J ;

Palucka, AK .

IMMUNE MECHANISMS AND DISEASE, 2003, 987 :180-187

[5]

A comparison of normalization methods for high density oligonucleotide array data based on variance and bias [J].

Bolstad, BM ;

Irizarry, RA ;

Åstrand, M ;

Speed, TP .

BIOINFORMATICS, 2003, 19 (02) :185-193

[6]

Selective suppression of IL-12 production by chemoattractants [J].

Braun, MC ;

Lahey, E ;

Kelsall, BL .

JOURNAL OF IMMUNOLOGY, 2000, 164 (06) :3009-3017

[7]

MONOCYTE CHEMOATTRACTANT PROTEIN-1 ACTS AS A T-LYMPHOCYTE CHEMOATTRACTANT [J].

CARR, MW ;

ROTH, SJ ;

LUTHER, E ;

ROSE, SS ;

SPRINGER, TA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (09) :3652-3656

[8]

Mechanisms of disease - The many roles of chemokines and chemokine receptors in inflammation [J].

Charo, IF ;

Ransohoff, RM .

NEW ENGLAND JOURNAL OF MEDICINE, 2006, 354 (06) :610-621

[9]

Real-time quantification of microRNAs by stem-loop RT-PCR [J].

Chen, CF ;

Ridzon, DA ;

Broomer, AJ ;

Zhou, ZH ;

Lee, DH ;

Nguyen, JT ;

Barbisin, M ;

Xu, NL ;

Mahuvakar, VR ;

Andersen, MR ;

Lao, KQ ;

Livak, KJ ;

Guegler, KJ .

NUCLEIC ACIDS RESEARCH, 2005, 33 (20) :e179.1-e179.9

[10]

Chensue SW, 1996, J IMMUNOL, V157, P4602

← 1 2 3 4 5 6 →