Increase of caspase-3 activity by lignans from Machilus thunbergii in HL-60 cells

被引：41

作者：

Park, BY

Min, BS

Kwon, OK

Oh, SR

Ahn, KS

Kim, TJ

Kim, DY

Bae, KW

Lee, HK ^{[1
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol, Taejon 305333, South Korea

[2] Chungnam Natl Univ, Coll Pharm, Taejon 305764, South Korea

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2004年 / 27卷 / 08期

关键词：

Machilus thunbergii; lignan; caspase-3; DNA fragmentation; apoptosis;

D O I：

10.1248/bpb.27.1305

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Nine lignans and two butanolides were isolated from the stem bark of Machilus thunbergii and their structures were identified as machilin A (1), licarin B (2), zuonin B (3), macelignan (4), secoisolancifolide (5), isolancifolide (6), oleiferin C (7), meso-dihydroguaiaretic acid (8), licarin A (9), machilin F (10), and nectandrin B (11) by spectroscopic means. These compounds were assessed for their abilities to activate a caspase-3 activity in human promyeloid leukemic HL-60 cells. The intracellular caspase-3 activity of macelignan (4), oleiferin C (7), meso-dihydroguaiaretic acid (8), and licarin A (9) increased approximately 3.04, 6.16, 2.10, and 3.10-fold at 100 mum over that of untreated control. In addition, compounds 4, 7, 8, and 9 induced internucleosomal DNA fragmentation in HL-60 cells.

引用

页码：1305 / 1307

页数：3

共 18 条

[1]

CHUNG BS, 1990, DICT KOREAN FOLK MED, P458

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