Expression of staphylococcal protein Sbi is induced by human IgG

被引：6

作者：

Zhang, LH ^{[1
]}

Rosander, A ^{[1
]}

Jacobsson, K ^{[1
]}

Lindberg, M ^{[1
]}

Frykberg, L ^{[1
]}

机构：

[1] Swedish Univ Agr Sci, Dept Microbiol, S-75007 Uppsala, Sweden

来源：

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 2000年 / 28卷 / 03期

关键词：

protein Sbi; IgG binding; Staphylococcus aureus; inducible expression; environment-sensing receptor;

D O I：

10.1016/S0928-8244(00)00158-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Protein Sbi is an IgG- and beta(2) glycoprotein I-binding protein on the surface of Staphylococcus aureus. In most strains, the amount of protein Sbi on the cell surface is very low under normal growth conditions. However, here we show that after growth in the presence of human serum, the amount is significantly increased. In S. aureus strain 8325-4, the observed increase is concentration-dependent and the highest level is found approximately 2 h after serum addition. The active molecule in serum was found to be IgG, which causes an increase of surface-located protein Sbi in S. aureus strain 8325-4, in the clinical isolates tested as well as in protein A-negative mutants. Thus, the results suggest that binding of IgG to protein Sbi upregulates protein Sbi synthesis. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：211 / 218

页数：8

共 25 条

[1] REGULATION OF EXOPROTEIN EXPRESSION IN STAPHYLOCOCCUS-AUREUS BY A LOCUS (SAR) DISTINCT FROM AGR [J].

CHEUNG, AL ;

KOOMEY, JM ;

BUTLER, CA ;

PROJAN, SJ ;

FISCHETTI, VA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (14) :6462-6466

[2] CLONING AND SEQUENCING OF SARA OF STAPHYLOCOCCUS-AUREUS, A GENE REQUIRED FOR THE EXPRESSION OF AGR [J].

CHEUNG, AL ;

PROJAN, SJ .

JOURNAL OF BACTERIOLOGY, 1994, 176 (13) :4168-4172

[3] Selective activation of sar promoters with the use of green fluorescent protein transcriptional fusions as the detection system in the rabbit endocarditis model [J].

Cheung, AL ;

Nast, CC ;

Bayer, AS .

INFECTION AND IMMUNITY, 1998, 66 (12) :5988-5993

[4]

CHONN A, 1992, J BIOL CHEM, V267, P18759

[5]

Cleary Patrick, 1994, Trends in Microbiology, V2, P131, DOI 10.1016/0966-842X(94)90600-9

[6] Staphylococcus aureus genetic loci impacting growth and survival in multiple infection environments [J].

Coulter, SN ;

Schwan, WR ;

Ng, EYW ;

Langhorne, MH ;

Ritchie, HD ;

Westbrock-Wadman, S ;

Hufnagle, WO ;

Folger, KR ;

Bayer, AS ;

Stover, CK .

MOLECULAR MICROBIOLOGY, 1998, 30 (02) :393-404

[7]

Easmon CSF, 1983, STAPHYLOCOCCI STAPHY

[8] SIMULTANEOUS IDENTIFICATION OF BACTERIAL VIRULENCE GENES BY NEGATIVE SELECTION [J].

HENSEL, M ;

SHEA, JE ;

GLEESON, C ;

JONES, MD ;

DALTON, E ;

HOLDEN, DW .

SCIENCE, 1995, 269 (5222) :400-403

[9]

JACOBSSON K, 1995, BIOTECHNIQUES, V18, P878

[10]

KRONVALL G, 1970, J IMMUNOL, V104, P140

← 1 2 3 →