Positive correlation between inhibitors of matrix metalloproteinase 1 and matrix metalloproteinases in malignant ovarian tumor tissues

被引:25
作者
Kikkawa, F [1 ]
Tamakoshi, K [1 ]
Nawa, A [1 ]
Shibata, K [1 ]
Yamagata, S [1 ]
Yamagata, T [1 ]
Suganuma, N [1 ]
机构
[1] TOKYO INST TECHNOL,DEPT BIOMOL ENGN,YOKOHAMA,KANAGAWA 227,JAPAN
关键词
matrix metalloproteinase; ovarian cancer; tissue inhibitor of metalloproteinase; zymography;
D O I
10.1016/S0304-3835(97)00295-4
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
To investigate the relation between matrix metalloproteinases (MMP) and tissue inhibitors of metalloproteinases (TIMP) in malignant ovarian tumor, MMP and TIMP activities in conditioned media of 16 malignant ovarian tumor tissues and six normal ovaries were detected by zymography and reverse zymography, respectively and were quantitated with a densitometer. TIMP-1 and TIMP-2 were detected in all normal and malignant ovarian tumor tissues by reverse zymography. In normal ovaries, the intensity of TIMP-2 bands was stronger than TIMP-1, but in malignant tumor tissues those of TIMP-1 were stronger, The ratios of TIMP-1 to TIMP-2 and MMP-9 to MMP-2 were significantly higher in malignant tumor tissues than in normal ovaries (P < 0.001), TIMP activity consisting of TIMP-1 and TIMP-2 correlated significantly to MMP activity of MMP-2 and MMP-9 (r = 0.67, P < 0.005). There was a significant correlation between TIMP-1 activity and MMP activity (P = 0.72, P < 0.001), but no correlation was observed between TIMP-2 activity and MMP activity. The high level of TIMP-1 appeared to be related to malignant phenotype in ovaries as well as the high level of MMP-9. (C) 1997 Elsevier Science Ireland Ltd.
引用
收藏
页码:109 / 115
页数:7
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