A highly sensitive and dynamic immunofluorescent cytometric bead assay for the detection of HIV-1 p24

被引：52

作者：

Biancotto, Angelique ^{[1
]}

Brichacek, Beda ^{[1
]}

Chen, Silvia S. ^{[1
]}

Fitzgerald, Wendy ^{[1
]}

Lisco, Andrea ^{[1
]}

Vanpouille, Christophe ^{[1
]}

Margolis, Leonid ^{[1
]}

Grivel, Jean-Charles ^{[1
]}

机构：

[1] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Sect Intercellular Interact, Program Phys Biol, Bethesda, MD 20892 USA

来源：

JOURNAL OF VIROLOGICAL METHODS | 2009年 / 157卷 / 01期

关键词：

HIV-1; p24; Detection; Multiplex assay; Diagnosis; VIRUS TYPE-1 RNA; ANTIGEN-ASSAY; PLASMA; ANTIBODIES; INFECTION; SPECIMENS;

D O I：

10.1016/j.jviromet.2008.11.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleic acid measurements are used to follow HIV-1 viral load in clinical applications while p24 ELISA is commonly used to monitor HIV-1 replication in research settings. Current ELISA assays are expensive and offer a narrow dynamic measurement range. This report describes a simple, sensitive and inexpensive bead-based assay offering a wide dynamic measurement range. This cytometric bead assay allows the detection of p24 concentrations over 4 orders of magnitude from less than 0.4 pg to up to 20,000 pg ml(-1) in a volume of 50 mu l and can be combined with other measurements. Published by Elsevier B.V.

引用

收藏

页码：98 / 101

页数：4

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