Magnetic-field stimulated DNA oxidation

被引：73

作者：

Wang, J ^{[1
]}

Kawde, AN ^{[1
]}

机构：

[1] New Mexico State Univ, Dept Chem & Biochem, Las Cruces, NM 88003 USA

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2002年 / 4卷 / 04期

基金：

美国国家卫生研究院;

关键词：

magnet; DNA; carbon-paste electrode; guanine oxidation;

D O I：

10.1016/S1388-2481(02)00311-9

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A reversible and cyclic magnetic-field stimulated DNA oxidation is described. Positioning an external magnet below the electrode attracts the DNA-functionalized magnetic particles to the surface, and stimulates the oxidation of the guanine nucleobases. Using a dual carbon-paste electrode assembly we demonstrate a spatially controlled DNA oxidation, with an 'ON/OFF' switching of the electron-transfer reaction upon relocating the external magnetic field. The process can be reversed and repeated upon switching the position of the magnet, with and without oxidation signals in the presence and absence of the magnetic field, respectively. We also demonstrate a 'magnetic' carbon-paste electrode, with an internal magnet, that collects the DNA-modified beads and stimulates the DNA oxidation process. The site-specific activation of the DNA oxidation holds promise for new DNA arrays and other genoelectronic applications. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：349 / 352

页数：4

共 10 条

[1] Magneto-switchable bioelectrocatalysis [J].

Hirsch, R ;

Katz, E ;

Willner, I .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (48) :12053-12054

[2] Magneto-switchable electrogenerated biochemiluminescence [J].

Ichia, LSH ;

Katz, E ;

Wasserman, J ;

Willner, I .

CHEMICAL COMMUNICATIONS, 2002, (02) :158-159

[3] OSCILLOGRAPHIC POLAROGRAPHY OF HIGHLY POLYMERIZED DEOXYRIBONUCLEIC ACID [J].

PALECEK, E .

NATURE, 1960, 188 (4751) :656-657

[4] DIFFERENTIAL-PULSE VOLTAMMETRIC DETERMINATION OF RNA AT THE PICOMOLE LEVEL IN THE PRESENCE OF DNA AND NUCLEIC-ACID [J].

PALECEK, E ;

FOJTA, M .

ANALYTICAL CHEMISTRY, 1994, 66 (09) :1566-1571

[5] Detecting DNA hybridization and damage [J].

Palecek, E ;

Fojta, M .

ANALYTICAL CHEMISTRY, 2001, 73 (03) :74A-83A

[6]

PALECEK E, IN PRESS TALANTA

[7] POLAROGRAPHY OF CIRCULAR DNAS [J].

VOJTISKOVA, M ;

LUKASOVA, E ;

JELEN, F ;

PALECEK, E .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1981, 8 (05) :487-496

[8] Adsorptive stripping potentiometry of DNA at electrochemically pretreated carbon paste electrodes [J].

Wang, J ;

Cai, XH ;

Jonsson, C ;

Balakrishnan, M .

ELECTROANALYSIS, 1996, 8 (01) :20-24

[9] DNA-modified electrode for the detection of aromatic amines [J].

Wang, J ;

Rivas, G ;

Luo, DB ;

Cai, XH ;

Valera, FS ;

Dontha, N .

ANALYTICAL CHEMISTRY, 1996, 68 (24) :4365-4369

[10] Magnetic bead-based label-free electrochemical detection of DNA hybridization [J].

Wang, J ;

Kawde, AN ;

Erdem, A ;

Salazar, M .

ANALYST, 2001, 126 (11) :2020-2024

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