The inhibitory effect of glucose on phosphoenolpyruvate carboxykinase gene expression in cultured hepatocytes is transcriptional and requires glucose metabolism

被引：29

作者：

Cournarie, F ^{[1
]}

Azzout-Marniche, D ^{[1
]}

Foretz, M ^{[1
]}

Guichard, C ^{[1
]}

Ferre, P ^{[1
]}

Foufelle, F ^{[1
]}

机构：

[1] Univ Paris 06, Inst Biomed Cordeliers, INSERM, U465, F-75270 Paris 06, France

来源：

FEBS LETTERS | 1999年 / 460卷 / 03期

关键词：

phosphoenolpyruvate carboxykinase; glucose; liver; transcription; glucose-6-phosphate;

D O I：

10.1016/S0014-5793(99)01407-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphoenolpyruvate carboxykinase (PEPCK) is the rate-limiting enzyme of gluconeogenesis in the liver. PEPCK gene expression is controlled at the transcriptional level and is mainly regulated by hormones that are involved in glucose homeostasis, In this study, we have investigated the role of glucose on PEPCK gene expression in cultured hepatocytes. We demonstrate that glucose counteracts the stimulatory effect of glucocorticoids and cAMP on PEPCK expression, Glucose must be metabolized through glucokinase to have its inhibitory effect, The effect of glucose is mainly transcriptional and the region responsible for glucose inhibition is localized in the first 490 bp of the promoter. (C) 1999 Federation of European Biochemical Societies.

引用

页码：527 / 532

页数：6

共 35 条

[1] HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS - A BIOCHEMICAL AND FINE STRUCTURAL STUDY
BERRY, MN
FRIEND, DS
[J]. JOURNAL OF CELL BIOLOGY, 1969, 43 (03) : 506 - +
[2] BRUN T, 1993, J BIOL CHEM, V268, P18905
[3] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[4] LIPOGENIC ENZYME-ACTIVITIES AND MESSENGER-RNA IN RAT ADIPOSE-TISSUE AT WEANING
COUPE, C
PERDEREAU, D
FERRE, P
HITIER, Y
NARKEWICZ, M
GIRARD, J
[J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 258 (01): : E126 - E133
[5] DOIRON B, 1994, J BIOL CHEM, V269, P10213
[6] Transcriptional glucose signaling through the glucose response element is mediated by the pentose phosphate pathway
Doiron, B
Cuif, MH
Chen, RH
Kahn, A
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (10) : 5321 - 5324
[7] Correction of diabetic alterations by glucokinase
Ferre, T
Pujol, A
Riu, E
Bosch, F
Valera, A
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (14) : 7225 - 7230
[8] Foretz M, 1999, MOL CELL BIOL, V19, P3760
[9] FORETZ M, 1999, IN PRESS P NATL ACAD
[10] FOUFELLE F, 1992, J BIOL CHEM, V267, P20543

← 1 2 3 4 →