GFP-tagged balancer chromosomes for Drosophila melanogaster

被引：56

作者：

Casso, D ^{[1
]}

Ramírez-Weber, FA ^{[1
]}

Kornberg, TB ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA

来源：

MECHANISMS OF DEVELOPMENT | 1999年 / 88卷 / 02期

关键词：

GFP; Drosophila; balancer chromosomes;

D O I：

10.1016/S0925-4773(99)00174-4

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We constructed green fluorescent protein (GFP)-expressing balancer chromosomes for each of the three major chromosomes of Drosophila melanogaster. Expression of GFP in these chromosomes is driven indirectly by a Kruppel (Kr) promoter, via the yeast GAL4-UAS regulatory system. GFP fluorescence can be seen in embryos as early as the germ band extension stage, and can also be seen in larvae, pupae, and adults. We show the patterns of GFP expression of these balancers and demonstrate the use of the balancers to identify homozygous progeny. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：229 / 232

页数：4

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