WAFL, a new protein involved in regulation of early endocytic transport at the intersection of actin and microtubule dynamics

被引：25

作者：

Viklund, Ing-Marie ^{[1
,2
]}

Aspenstrom, Pontus ^{[1
,3
]}

Meas-Yedid, Vannary ^{[4
]}

Zhang, Bo ^{[4
]}

Kopec, Jolanta ^{[2
,5
]}

Agren, Daniel ^{[2
,5
]}

Schneider, Gunter ^{[2
,5
]}

D'Amato, Mauro ^{[1
,2
]}

Olivo-Marin, Jean-Christophe ^{[4
]}

Sansonetti, Philippe ^{[6
]}

Van Nhieu, Guy Tran ^{[6
]}

Pettersson, Sven ^{[1
,2
,7
]}

机构：

[1] Karolinska Inst, Dept Microbiol Tumor & Cell Biol, S-17177 Stockholm, Sweden

[2] Karolinska Inst, Strateg Res Ctr IRIS, S-17177 Stockholm, Sweden

[3] Uppsala Univ, Biomed Ctr, Ludwig Inst Canc Res, Uppsala, Sweden

[4] Inst Pasteur, Unite Anal Images Quantitat, Paris, France

[5] Karolinska Inst, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden

[6] Inst Pasteur, Unite Pathogenie Microbienne Mol, Paris, France

[7] Genome Inst Singapore, Singapore, Singapore

来源：

EXPERIMENTAL CELL RESEARCH | 2009年 / 315卷 / 06期

基金：

瑞典研究理事会;

关键词：

Endocytosis; Transport; Actin; Microtubules; KIAA0674; FKBP15; FKBP133; WIP; ALDRICH-SYNDROME PROTEIN; N-WASP; WIP; LYSOSOMES; COMPLEX; POLYMERIZATION; TRAFFICKING; ENDOSOMES; MEMBRANE; MOVEMENT;

D O I：

10.1016/j.yexcr.2008.12.004

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We have previously identified a new gene with sequence homology to the WASP-family of actin regulators denoted WAFL (WASP and FKBP-like). Here we report a possible biological function for WAFL, by demonstrating an association to early endosomes via its central coiled-coil domain, Further we show by functional and structural studies that WAFL is associated with both microtubules and the actin filament system, the two means of transport of early endosomes. In addition, WAFL interacts with WASP-interacting protein (WIP) and actin, thus linking WAFL to actin dynamics. The use of RNAi depletion of WAFL shows that WAFL-deficient cells display delayed transport of endosomal cargo. Our findings are compatible with a model whereby WAFL is involved in the transport of early endosomes at the level of transition between microfilament-based and microtubule-based movement. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：1040 / 1052

页数：13

共 30 条

[1] Transferrin recycling and dextran transport to lysosomes is differentially affected by bafilomycin, nocodazole, and low temperature [J].

Baravalle, G ;

Schober, D ;

Huber, M ;

Bayer, N ;

Murphy, RF ;

Fuchs, R .

CELL AND TISSUE RESEARCH, 2005, 320 (01) :99-113

[2] Phosphatidylinositol 4,5-biphosphate (PIP2)-induced vesicle movement depends on N-WASP and involves Nck, WIP, and Grb2 [J].

Benesch, S ;

Lommel, S ;

Steffen, A ;

Stradal, TEB ;

Scaplehorn, N ;

Way, M ;

Wehland, J ;

Rottner, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (40) :37771-37776

[3]

Benjamini Y, 2001, ANN STAT, V29, P1165

[4] CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].

BENJAMINI, Y ;

HOCHBERG, Y .

JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300

[5] Endocytic delivery to lysosomes mediated by concurrent fusion and kissing events in living cells [J].

Bright, NA ;

Gratian, MJ ;

Luzio, JP .

CURRENT BIOLOGY, 2005, 15 (04) :360-365

[6] Differential distribution of dynamin isoforms in mammalian cells [J].

Cao, H ;

Garcia, F ;

McNiven, MA .

MOLECULAR BIOLOGY OF THE CELL, 1998, 9 (09) :2595-2609

[7] Microtubule motors at the intersection of trafficking and transport [J].

Caviston, Juliane P. ;

Holzbaur, Erika L. F. .

TRENDS IN CELL BIOLOGY, 2006, 16 (10) :530-537

[8] Regulated portals of entry into the cell [J].

Conner, SD ;

Schmid, SL .

NATURE, 2003, 422 (6927) :37-44

[9] Coassembly of flotillins induces formation of membrane microdomains, membrane curvature, and vesicle budding [J].

Frick, Manfred ;

Bright, Nicholas A. ;

Riento, Kirsi ;

Bray, Aurelie ;

Merrified, Christien ;

Nichols, Benjamin J. .

CURRENT BIOLOGY, 2007, 17 (13) :1151-1156

[10] Differential cytoskeletal changes during growth cone collapse in response to hSema III and thrombin [J].

Fritsche, J ;

Reber, BFX ;

Schindelholz, B ;

Bandtlow, CE .

MOLECULAR AND CELLULAR NEUROSCIENCE, 1999, 14 (4-5) :398-418

← 1 2 3 →