Characterization of a new member of the TNF family expressed on antigen presenting cells

被引:90
作者
Tribouley, C [1 ]
Wallroth, M [1 ]
Chan, V [1 ]
Paliard, X [1 ]
Fang, E [1 ]
Lamson, G [1 ]
Pot, D [1 ]
Escobedo, J [1 ]
Williams, LT [1 ]
机构
[1] Chiron Corp, Emeryville, CA 94608 USA
关键词
antigen presenting cells; apoptosis; immune response; inflammation; LPS; PBMC; T cell; TNF;
D O I
10.1515/BC.1999.186
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The TNF family is involved in the regulation of the immune system, and its members have been implicated in a variety of biological events such as apoptosis, cell proliferation, differentiation and survival. Here we present a new member of the TNF family, tumor necrosis factor super family member 20 (TNFSF20) that we have identified from the expressed sequence tag (EST) database and characterized. The human protein is a 285 amino acid long type II transmembrane protein and is 19% homologous to TNF in its extra-cellular domain. TNFSF20 is expressed at the surface of antigen presenting cells such as cells of the macrophage-monocyte lineage and dendritic cells. After treatment with bacterial lipopolysaccharide (LPS), TNFSF20 expression is downregulated at the surface of the expressing cells, suggesting that the membrane-bound protein gets cleaved, and that a soluble factor is released in the extra-cellular compartment. The soluble form of the recombinant TNFSF20 induces proliferation of resting peripheral blood monocytes (PBMC) and cell death of activated lymphocytes. TNFSF20 might therefore play a critical role in the regulation of cell-mediated immune responses.
引用
收藏
页码:1443 / 1447
页数:5
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