The caudal homeobox protein cdx-2/3 activates endogenous proglucagon gene expression in InR1-G9 islet cells

被引：39

作者：

Jin, TR ^{[1
]}

Trinh, DKY ^{[1
]}

Wang, F ^{[1
]}

Drucker, DJ ^{[1
]}

机构：

[1] UNIV TORONTO, TORONTO HOSP, DEPT MED, BANTING & BEST DIABET CTR, TORONTO, ON M5G 2C4, CANADA

来源：

MOLECULAR ENDOCRINOLOGY | 1997年 / 11卷 / 02期

关键词：

D O I：

10.1210/me.11.2.203

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The proglucagon gene is expressed in a highly cell-specific manner in islet and enteroendocrine cells. DNA sequences within the proximal proglucagon G1 promoter region bind the homeobox protein cdx-2/3, and cdx-2/3 activates the proglucagon promoter in fibroblasts. We show here that cdx-2/3 activates the proglucagon promoter in both islet (InR1-G9) and enteroendocrine (STC-1 and GLUTag) cell lines, Furthermore, transfected cdx-2/3 increased the levels of endogenous proglucagon mRNA transcripts in both transient and stable transfections of InR1-G9 islet cells. The cdx-2/3-dependent induction of endogenous proglucagon mRNA transcripts in stable islet lines was associated with a corresponding increase in the transcriptional activity of proglucagon promoter-luciferase plasmids, An amino-terminally truncated cdx-2/3 derivative containing the homeodomain and carboxy-terminal region of the molecule inhibited both the cdx-2/3 activation of the proglucagon promoter and the induction of endogenous proglucagon mRNA transcripts, These observations demonstrate that cdx-2/3, acting through the proximal G1 element, is a major transcriptional determinant of cell-specific proglucagon gene expression in pancreatic islet cells.

引用

页码：203 / 209

页数：7

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