Role of the ptsN gene product in catabolite repression of the Pseudomonas putida TOL toluene degradation pathway in chemostat cultures

被引：27

作者：

Aranda-Olmedo, Isabel ^{[1
]}

Marin, Patricia ^{[1
]}

Ramos, Juan L. ^{[1
]}

Marques, Silvia ^{[1
]}

机构：

[1] CSIC, Estac Expt Zaidin, Dept Environm Protect, E-18008 Granada, Spain

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2006年 / 72卷 / 11期

关键词：

D O I：

10.1128/AEM.01067-06

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Pseudomonas putida KT2440 TOL upper pathway is repressed under nonlimiting conditions in cells growing in chemostat with succinate as a carbon source. We show that the ptsN gene product IIA(Ntr) participates in this repression. Crc, involved in yeast extract-dependent repression in batch cultures, did not influence expression when cells were growing in a chemostat with succinate at maximum rate.

引用

页码：7418 / 7421

页数：4

共 26 条

[1] REGULATOR AND ENZYME SPECIFICITIES OF THE TOL PLASMID-ENCODED UPPER PATHWAY FOR DEGRADATION OF AROMATIC-HYDROCARBONS AND EXPANSION OF THE SUBSTRATE RANGE OF THE PATHWAY [J].

ABRIL, MA ;

MICHAN, C ;

TIMMIS, KN ;

RAMOS, JL .

JOURNAL OF BACTERIOLOGY, 1989, 171 (12) :6782-6790

[2] Integration of signals through Crc and PtsN in catabolite repression of Pseudomonas putida TOL plasmid pWW0 [J].

Aranda-Olmedo, I ;

Ramos, JL ;

Marqués, S .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (08) :4191-4198

[3] Activation of the toluene-responsive regulator XyIR causes a transcriptional switch between σ54 and σ70 promoters at the divergent Pr/Ps region of the TOL plasmid [J].

Bertoni, G ;

Marqués, S ;

de Lorenzo, V .

MOLECULAR MICROBIOLOGY, 1998, 27 (03) :651-659

[4] The IIANtr (PtsN) protein of Pseudomonas putida mediates the C source inhibition of the σ54-dependent Pu promoter of the TOL plasmid [J].

Cases, I ;

Pérez-Martín, J ;

de Lorenzo, V .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (22) :15562-15568

[5] Involvement of sigma(54) in exponential silencing of the Pseudomonas putida TOL plasmid Pu promoter [J].

Cases, I ;

deLorenzo, V ;

PerezMartin, J .

MOLECULAR MICROBIOLOGY, 1996, 19 (01) :7-17

[6] EARLY AND LATE RESPONSES OF TOL PROMOTERS TO PATHWAY INDUCERS - IDENTIFICATION OF POSTEXPONENTIAL PROMOTERS IN PSEUDOMONAS-PUTIDA WITH LACZ-TET BICISTRONIC REPORTERS [J].

DELORENZO, V ;

CASES, I ;

HERRERO, M ;

TIMMIS, KN .

JOURNAL OF BACTERIOLOGY, 1993, 175 (21) :6902-6907

[7] Expression of the Pseudomonas putida OCT plasmid alkane degradation pathway is modulated by two different global control signals:: Evidence from continuous cultures [J].

Dinamarca, MA ;

Aranda-Olmedo, I ;

Puyet, A ;

Rojo, F .

JOURNAL OF BACTERIOLOGY, 2003, 185 (16) :4772-4778

[8] STABILITY OF TOL PLASMID PWW0 IN PSEUDOMONAS-PUTIDA MT-2 UNDER NONSELECTIVE CONDITIONS IN CONTINUOUS CULTURE [J].

DUETZ, WA ;

VANANDEL, JG .

JOURNAL OF GENERAL MICROBIOLOGY, 1991, 137 :1369-1374

[9] Catabolite repression of the toluene degradation pathway in Pseudomonas putida harboring pWWO under various conditions of nutrient limitation in chemostat culture [J].

Duetz, WA ;

Marques, S ;

Wind, B ;

Ramos, JL ;

vanAndel, JG .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (02) :601-606

[10] INDUCIBILITY OF THE TOL CATABOLIC PATHWAY IN PSEUDOMONAS-PUTIDA (PWW0) GROWING ON SUCCINATE IN CONTINUOUS-CULTURE - EVIDENCE OF CARBON CATABOLITE REPRESSION CONTROL [J].

DUETZ, WA ;

MARQUES, S ;

DEJONG, C ;

RAMOS, JL ;

VANANDEL, JG .

JOURNAL OF BACTERIOLOGY, 1994, 176 (08) :2354-2361

← 1 2 3 →