Glucose biosensing based on the highly efficient immobilization of glucose oxidase on a Prussian blue modified nanostructured Au surface

被引：45

作者：

Ahmadalinezhad, Asieh ^{[1
]}

Kafi, A. K. M. ^{[1
]}

Chen, Aicheng ^{[1
]}

机构：

[1] Lakehead Univ, Dept Chem, Thunder Bay, ON P7B 5E1, Canada

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2009年 / 11卷 / 10期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Glucose; Nanostructured Au; Glucose oxidase; Biosensor; Prussian blue; GOLD NANOPARTICLES; ELECTRODE; SENSOR; POLYMER;

D O I：

10.1016/j.elecom.2009.08.048

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

We report on a novel glucose biosensor based on the immobilization of glucose oxidase (GOx) on a Prussian blue modified nanoporous gold surface. The amperometric glucose biosensor fabricated in this study exhibits a fast response and the very low detection limit of 2.5 mu M glucose. The sensitivity of the biosensor was found to be very high, 177 mu A/mM; the apparent Michaelis-Menten constant is calculated to be 2.1 mM. In addition, the biosensor has good reproducibility and remains stable over 60 days. The anti-interference ability of the biosensor was also assessed, showing little interference from possible interferents such as ascorbic acid (AA), acetaminophen (AP) and uric acid (UA). (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：2048 / 2051

页数：4

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