A panel of 223 faecal samples was analysed to determine the clinical utility of the Seeplex (R) Diarrhea ACE Detection multiplex PCR system (Seeplex system; Seegene, Korea), a qualitative multiplexing PCR technology that enables simultaneous multi-pathogen detection of four viruses and/or ten bacteria associated with acute gastroenteritis. Conventional diagnostic methods and a norovirus-specific multiplex real-time RT-PCR detected 98 pathogens in 96 samples. The Seeplex system detected 81 pathogens in 75 samples. All samples positive for adenovirus, norovirus, Campylobacter spp., Escherichia coli O157, Shigella spp. or Vibrio spp. were detected by the Seeplex system. Rotavirus, Clostridium difficile toxin B, and Salmonella spp. were not detected in 12.5%, 50% and 15.8% of samples, respectively. Additional multiple infections were detected in 19 samples by the Seeplex system. The Seeplex system provides significant additional diagnostic capability for the syndromic diagnosis of acute gastroenteritis with increased sensitivity for the majority of pathogens.
机构:
Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Cunningham, Scott A.
Sloan, Lynne M.
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Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Sloan, Lynne M.
Nyre, Lisa M.
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机构:
Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Nyre, Lisa M.
Vetter, Emily A.
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Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Vetter, Emily A.
Mandrekar, Jayawant
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Mayo Clin, Div Biomed Stat & Informat, Dept Hlth Sci Res, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Mandrekar, Jayawant
Patel, Robin
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机构:
Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Mayo Clin, Div Infect Dis, Dept Internal Med, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
机构:
NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA
Ctr Dis Control & Prevent, Div Viral Dis, Natl Ctr Immunizat & Resp Dis, Atlanta, GA USANIH, Fogarty Int Ctr, Bethesda, MD 20892 USA
Glass, Roger I.
Parashar, Umesh D.
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机构:
Ctr Dis Control & Prevent, Div Viral Dis, Natl Ctr Immunizat & Resp Dis, Atlanta, GA USANIH, Fogarty Int Ctr, Bethesda, MD 20892 USA
Parashar, Umesh D.
Estes, Mary K.
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机构:
Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USANIH, Fogarty Int Ctr, Bethesda, MD 20892 USA
机构:
Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Cunningham, Scott A.
Sloan, Lynne M.
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h-index: 0
机构:
Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Sloan, Lynne M.
Nyre, Lisa M.
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h-index: 0
机构:
Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Nyre, Lisa M.
Vetter, Emily A.
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h-index: 0
机构:
Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Vetter, Emily A.
Mandrekar, Jayawant
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h-index: 0
机构:
Mayo Clin, Div Biomed Stat & Informat, Dept Hlth Sci Res, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Mandrekar, Jayawant
Patel, Robin
论文数: 0引用数: 0
h-index: 0
机构:
Mayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Mayo Clin, Div Infect Dis, Dept Internal Med, Rochester, MN 55905 USAMayo Clin, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
机构:
NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA
Ctr Dis Control & Prevent, Div Viral Dis, Natl Ctr Immunizat & Resp Dis, Atlanta, GA USANIH, Fogarty Int Ctr, Bethesda, MD 20892 USA
Glass, Roger I.
Parashar, Umesh D.
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Dis Control & Prevent, Div Viral Dis, Natl Ctr Immunizat & Resp Dis, Atlanta, GA USANIH, Fogarty Int Ctr, Bethesda, MD 20892 USA
Parashar, Umesh D.
Estes, Mary K.
论文数: 0引用数: 0
h-index: 0
机构:
Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USANIH, Fogarty Int Ctr, Bethesda, MD 20892 USA