Parainfluenza virus type 5 (PIV-5) morphology revealed by cryo-electron microscopy

被引：19

作者：

Terrier, Olivier ^{[1
,2
,3
]}

Rolland, Jean-Paul ^{[4
]}

Rosa-Calatrava, Manuel ^{[1
,2
,3
]}

Lina, Bruno ^{[1
,2
,3
,5
]}

Thomas, Daniel ^{[4
]}

Moules, Vincent ^{[1
,2
,3
]}

机构：

[1] Univ Lyon, F-69000 Lyon, France

[2] Univ Lyon 1, Fac Med RTH Laennec, F-69622 Villeurbanne, France

[3] CNRS, FRE VirPath 3011, F-69008 Lyon, France

[4] Univ Rennes 1, CNRS Interact Cellulaires & Mol, UMR6026, F-35042 Rennes, France

[5] Hosp Civils Lyon, Ctr Biol & Pathol Est, Virol Lab, F-69677 Bron, France

来源：

VIRUS RESEARCH | 2009年 / 142卷 / 1-2期

关键词：

Paramyxoviridae; Parainfluenza; Cryo-electron microscopy; Virus structure; ELECTRON CRYOMICROSCOPY; MEMBRANE-FUSION; HN-PROTEINS; VIRIONS; CELLS; SV5;

D O I：

10.1016/j.virusres.2008.12.017

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The knowledge of parainfluenza type 5 (PIV-5) virion morphology is essentially based on the observation of negatively stained preparations in conventional transmission electron microscopy (CTEM). In this study, the ultrastructure of frozen-hydrated intact PIV-5 was examined by cryo-electron microscopy (cryo-EM). Cryo-EM revealed a majority of spherical virions (70%), with a lower pleiomorphy than originally observed in CTEM. Phospholipid bilayer thickness, spike length and glycoprotein spikes density were measured. About 2000 glycoprotein spikes were present in an average-sized spherical virion. Altogether, these data depict a more precise view of PIV-5 morphology. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：200 / 203

页数：4

共 19 条

[1] MORPHOLOGY OF SV5 VIRUS [J].