Genetic architecture of the polyketide synthases for methymycin and pikromycin series macrolides

被引:25
作者
Xue, YQ
Wilson, D
Sherman, DH [1 ]
机构
[1] Univ Minnesota, Dept Microbiol, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Biol Proc Technol Inst, Minneapolis, MN 55455 USA
关键词
antibiotics; gene cluster; ketolide; modular PKS;
D O I
10.1016/S0378-1119(00)00003-2
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The methymycin and pikromycin series of antibiotics are structurally related macrolides produced by several Streptomyces species, including Streptomyces venezuelae ATCC 15439, which produces both 12-membered ring macrolides methymycin, neomethymycin, and 14-membered ring macrolides pikromycin and narbomycin. Cloning and sequencing of the biosynthetic gene clusters for these macrolides from three selected Streptomyces strains revealed a common genetic architecture of their polyketide syntheses (PKSs). Unlike PKS clusters of other 14-membered ring macrolides such as erythromycin and oleandomycin, each of the pikromycin series producers harbors a six module PKS cluster, in which modules 5 and 6 are encoded on two separate proteins instead of one bimodular protein, as well as a thioesterase II gene immediately downstream of the main PKS gene. The results shed new light on the evolution of modular PKSs and provide further evidence on the regulation of methymycin and pikromycin production in S. venezuelae ATCC 15439. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:203 / 211
页数:9
相关论文
共 31 条
  • [1] Synthesis and antibacterial activity of ketolides (6-O-methyl-3-oxoerythromycin derivatives):: A new class of antibacterials highly potent against macrolide-resistant and -susceptible respiratory pathogens
    Agouridas, C
    Denis, A
    Auger, JM
    Benedetti, Y
    Bonnefoy, A
    Bretin, F
    Chantot, JF
    Dussarat, A
    Fromentin, C
    D'Ambrières, SG
    Lachaud, S
    Laurin, P
    Le Martret, O
    Loyau, V
    Tessot, N
    [J]. JOURNAL OF MEDICINAL CHEMISTRY, 1998, 41 (21) : 4080 - 4100
  • [2] THE RELATIONSHIP BETWEEN BASE COMPOSITION AND CODON USAGE IN BACTERIAL GENES AND ITS USE FOR THE SIMPLE AND RELIABLE IDENTIFICATION OF PROTEIN-CODING SEQUENCES
    BIBB, MJ
    FINDLAY, PR
    JOHNSON, MW
    [J]. GENE, 1984, 30 (1-3) : 157 - 166
  • [3] A chain initiation factor common to both modular and aromatic polyketide synthases
    Bisang, C
    Long, PF
    Cortés, J
    Westcott, J
    Crosby, J
    Matharu, AL
    Cox, RJ
    Simpson, TJ
    Staunton, J
    Leadlay, PF
    [J]. NATURE, 1999, 401 (6752) : 502 - 505
  • [4] ANTIBIOTICA AUS ACTINOMYCCTEN .6. PIKROMYCIN, EIN BITTER SCHMECKENDES ANTIBIOTICUM AUS ACTINOMYCETEN
    BROCKMANN, H
    HENKEL, W
    [J]. CHEMISCHE BERICHTE-RECUEIL, 1951, 84 (03): : 284 - 288
  • [5] Impact of thioesterase activity on tylosin biosynthesis in Streptomyces fradiae
    Butler, AR
    Bate, N
    Cundliffe, E
    [J]. CHEMISTRY & BIOLOGY, 1999, 6 (05): : 287 - 292
  • [6] MACROLIDE BIOSYNTHESIS .7. INCORPORATION OF POLYKETIDE CHAIN ELONGATION INTERMEDIATES INTO METHYMYCIN
    CANE, DE
    LAMBALOT, RH
    PRABHAKARAN, PC
    OTT, WR
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (02) : 522 - 526
  • [7] CORBAZ R, 1955, NARBOMYCIN HELV CHIM, V38, P935
  • [8] MODULAR ORGANIZATION OF GENES REQUIRED FOR COMPLEX POLYKETIDE BIOSYNTHESIS
    DONADIO, S
    STAVER, MJ
    MCALPINE, JB
    SWANSON, SJ
    KATZ, L
    [J]. SCIENCE, 1991, 252 (5006) : 675 - 679
  • [9] DONIN MN, 1953, ANTIBIOT ANNU, V1, P179
  • [10] Dissecting and exploiting intermodular communication in polyketide synthases
    Gokhale, RS
    Tsuji, SY
    Cane, DE
    Khosla, C
    [J]. SCIENCE, 1999, 284 (5413) : 482 - 485