Limitation of a localized surface plasmon resonance sensor for Salmonella detection

被引：26

作者：

Fu, Junxue ^{[1
,2
]}

Park, Bosoon ^{[3
]}

Zhao, Yiping ^{[1
,2
]}

机构：

[1] Univ Georgia, Nanoscale Sci & Engn Ctr, Athens, GA 30602 USA

[2] Univ Georgia, Dept Phys & Astron, Athens, GA 30602 USA

[3] USDA ARS, Russell Res Ctr, Athens, GA 30605 USA

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2009年 / 141卷 / 01期

基金：

美国国家科学基金会;

关键词：

Localized surface plasmon resonance sensor; Salmonella detection; Au nanoparticle; LABEL-FREE DETECTION; RAPID DETECTION; QUANTITATIVE INTERPRETATION; ANTIGEN-ANTIBODY; BIOSENSOR; TYPHIMURIUM; DEPENDENCE; ELISA; BEADS;

D O I：

10.1016/j.snb.2009.06.020

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We have designed a localized surface plasmon resonance (LSPR) biosensor to perform whole cell detection of Salmonella using Au nanoparticles fabricated by an oblique angle deposition technique. The LSPR sensor shows a plasmon peak shift due to the Salmonella antigen and anti-Salmonella antibody reaction as verified by scanning electron microscopy and fluorescence microscopy. However, this shift is not sensitive to the concentration of the bacteria. We have modeled this detection system by means of Mie theory and effective medium theory, and find that due to the small contact area between the nanoparticle and the bacteria and the short range interaction of the local electric field, the plasmon peak shift induced by such a system is about 2-4 nm, regardless of the concentration of the bacteria. This is consistent with the experiments, and an alternative method is needed if the LSPR sensor is used for whole cell bacteria detection. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：276 / 283

页数：8

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