Cloning and sequencing of the cDNA encoding for a Na+/H+ exchanger from Xenopus laevis oocytes (X1-NHE)

被引：15

作者：

Busch, S

机构：

[1] Institut für Pharmakologie, Universitätsklinikum Essen, D-45122 Essen

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1997年 / 1325卷 / 01期

关键词：

Na+/H+ exchange; X-laevis; oocyte; cloning; cDNA;

D O I：

10.1016/S0005-2736(97)00011-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned and sequenced the cDNA for a Na+/H+ exchanger(NHE) from Xenopus laevis oocytes. This cDNA contains an open reading frame encoding a protein of 782 amino acids with 12 putative transmembrane domains and a long cytoplasmic tail. The protein exhibits a strong homology at the amino acid level to the human NHE-1 as well as to the beta NHE from trout red blood cells: 69% and 58% respectively. Two potential N-linked glycosylation sites at Asn(56) and Asn(351) were identified. Three potential protein kinase C phosphorylation sites at the cytoplasmic tail were identified at Ser(494), Thr(726) and Ser(747). RT-PCR revealed the expression of the X1-NHE in Xenopus heart, reticulocytes and skeletal muscle. (C) 1997 Elsevier Science B.V.

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页码：13 / 16

页数：4

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