Development and Characterization of Monoclonal Antibodies Specific for Mouse and Human Fcγ Receptors

被引：37

作者：

Tutt, Alison L. ^{[1
]}

James, Sonya ^{[1
]}

Laversin, Stephanie A. ^{[1
]}

Tipton, Thomas R. W. ^{[1
]}

Ashton-Key, Margaret ^{[1
]}

French, Ruth R. ^{[1
]}

Hussain, Khiyam ^{[1
]}

Vaughan, Andrew T. ^{[1
]}

Dou, Lang ^{[1
]}

Earley, Alexander ^{[1
]}

Dahal, Lekh N. ^{[1
]}

Lu, Chen ^{[1
]}

Dunscombe, Melanie ^{[1
]}

Chan, H. T. Claude ^{[1
]}

Penfold, Christine A. ^{[1
]}

Kim, Jinny H. ^{[1
]}

Potter, Elizabeth A. ^{[1
]}

Mockridge, C. Ian ^{[1
]}

Roghanian, Ali ^{[1
]}

Oldham, Robert J. ^{[1
]}

Cox, Kerry L. ^{[1
]}

Lim, Sean H. ^{[1
]}

Teige, Ingrid ^{[2
]}

Frendeus, Bjorn ^{[2
]}

Glennie, Martin J. ^{[1
]}

Beers, Stephen A. ^{[1
]}

Cragg, Mark S. ^{[1
]}

机构：

[1] Univ Southampton, Southampton Gen Hosp, Fac Med, Canc Sci Unit,Antibody & Vaccine Grp, Southampton SO16 6YD, Hants, England

[2] BioInvent Int, S-22362 Lund, Sweden

来源：

JOURNAL OF IMMUNOLOGY | 2015年 / 195卷 / 11期

关键词：

COPY-NUMBER VARIATION; ANTITUMOR ACTIVITIES; ENDOTHELIAL-CELLS; RIIB CD32B; GENE; IGG; EXPRESSION; THERAPY; INTERNALIZATION; AFFINITY;

D O I：

10.4049/jimmunol.1402988

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

Fc gamma Rs are key regulators of the immune response, capable of binding to the Fc portion of IgG Abs and manipulating the behavior of numerous cell types. Through a variety of receptors, isoforms, and cellular expression patterns, they are able to fine-tune and direct appropriate responses. Furthermore, they are key determinants of mAb immunotherapy, with mAb isotype and Fc gamma R interaction governing therapeutic efficacy. Critical to understanding the biology of this complex family of receptors are reagents that are robust and highly specific for each receptor. In this study, we describe the development and characterization of mAb panels specific for both mouse and human Fc gamma R for use in flow cytometry, immunofluorescence, and immunocytochemistry. We highlight key differences in expression between the two species and also patterns of expression that will likely impact on immunotherapeutic efficacy and translation of therapeutic agents from mouse to clinic.

引用

收藏

页码：5503 / 5516

页数：14

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