Phase variation analysis of Coxiella burnetii during serial passage in cell culture by use of monoclonal antibodies

被引：28

作者：

Hotta, A ^{[1
]}

Kawamura, M ^{[1
]}

To, H ^{[1
]}

Andoh, M ^{[1
]}

Yamaguchi, T ^{[1
]}

Fukushi, H ^{[1
]}

Hirai, K ^{[1
]}

机构：

[1] Gifu Univ, Fac Agr, Dept Vet Microbiol, Gifu 5011193, Japan

来源：

INFECTION AND IMMUNITY | 2002年 / 70卷 / 08期

关键词：

D O I：

10.1128/IAI.70.8.4747-4749.2002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Antigenic changes in Coxiella burnetii Nine Mile strain phase I during serial passages in cell culture were analyzed with three groups of monoclonal antibodies (MAbs) against lipopolysaccharide. The MAbs of group 1 did not react with organisms that were passaged over five times, and the MAbs of group 2 did not react with organisms that were passaged over eight times. The MAbs of group 3 reacted with organisms passaged up to 15 times but did not react with phase II cells. These results suggest that C burnetii could be diferentiated into four phase states during phase variation.

引用

页码：4747 / 4749

页数：3

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