No effect of MDMA (ecstasy) on cell death and 5-HT2A receptor density in organotypic rat hippocampal cultures

被引：9

作者：

Sveen, ML ^{[1
]}

Knudsen, GM ^{[1
]}

Aznar, S ^{[1
]}

机构：

[1] Copenhagen Univ Hosp, Neurobiol Res Unit, Unit 9201, DK-2100 Copenhagen, Denmark

来源：

NEUROSCIENCE LETTERS | 2004年 / 362卷 / 01期

关键词：

3,4 methylenedioxy-metamphetamine; in vitro; organotypic cultures; neurotoxicity; serotonin receptor; hippocampus;

D O I：

10.1016/j.neulet.2004.01.051

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

MDMA (3,4 Methylenedioxy-methamphetamine) binds and blocks the presynaptic serotonin reuptake transporters and postsynaptic serotonin 5-HT2A receptors, with highest affinity for the first. Whether 5-HT2A receptor density decreases due to MDMA's direct effect on postsynaptic serotonin receptors is at present not known. This study analyzes whether direct stimulation of the postsynaptic 5-HT2A receptor by MDMA in organotypic hippocampal cultures results in cell death and downregulation of this receptor. Fifty or 100 muM MDMA was added to I week old cultures, made of I I day old rat pups. Fluorojade and immunostaining for MAP2 and 5-HT2A to determine neurodegeneration, and changes in receptor density, respectively, resulted in no significant differences. MDMA's neurotoxicity and regulation of post-synaptic 5-HT2A receptors thus seems to require the presence of intact serotonergic terminals. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

引用

页码：6 / 9

页数：4

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