Generation and photosensitization properties of the oxidized radical of riboflavin: a laser flash photolysis study

被引：77

作者：

Lu, CY ^{[1
]}

Wang, WF ^{[1
]}

Lin, WZ ^{[1
]}

Han, ZH ^{[1
]}

Yao, SD ^{[1
]}

Lin, NY ^{[1
]}

机构：

[1] Acad Sinica, Shanghai Inst Nucl Res, Lab Radiat Chem, Shanghai 201800, Peoples R China

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 1999年 / 52卷 / 1-3期

基金：

中国国家自然科学基金;

关键词：

laser flash photolysis; electron transfer; riboflavin; nucleotides; photosensitization;

D O I：

10.1016/S1011-1344(99)00111-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using 248 nm laser flash photolysis, it has been demonstrated that the neutral oxidized radical of riboflavin (RF(-H)(.)) resulting from rapid deprotonation of the radical cation (RF.+) can be generated via monophotonic ionization when it is directly excited with a 248 nm laser and via oxidation by the SO4.- radical in aqueous solution. The RF(-H)(.) or RF.+ reacts with both purine nucleotides and pyrimidine nucleotides via electron transfer to give the radical cations of nucleotides. These results suggest that the reactions of oxidized riboflavin radical might be involved in DNA photosensitization in vivo and in vitro. (C) 1999 Elsevier Science S.A. All rights reserved.

引用

页码：111 / 116

页数：6

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