Tyrosinase isoforms from the fruitbodies of Agaricus bisporus

被引：72

作者：

Wichers, HJ

Gerritsen, YAM

Chapelon, CGJ

机构：

[1] Agrotechnological Res. Inst., 6708 PD Wageningen

来源：

PHYTOCHEMISTRY | 1996年 / 43卷 / 02期

关键词：

Agaricus bisporus; Agaricaceae; polyphenol oxidase; purification; tyrosinase;

D O I：

10.1016/0031-9422(96)00309-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Two monomeric tyrosinases were isolated from fruitbodies of Agaricus bisporus strain U1. Both tyrosinases; with pIs ca 5.2 and 5.1, showed an M(r) of ca 43 kDa under reducing and denaturing conditions and of ca 47 kDa under native conditions, and similar cresolase and catecholase activities of, respectively, 90-120 nkat mg(-1) and 17-18 mu kat mg(-1). The enzyme preparations were >95% homogeneous. Neither isoform appeared to be glycosylated or phosphorylated. Upon purification the isoforms showed a gradual decrease in pl from ca 5.4-5.6 to ca 5.1-5.2. Copyright (C) 1996 Elsevier Science Ltd.

引用

页码：333 / 337

页数：5

共 14 条

[1]

ANDREWS AT, 1986, ELECTROPHORESIS THEO, P37

[2]

BOUCHILLOUX S, 1963, J BIOL CHEM, V238, P1699

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4]

THE LOW-ISOELECTRIC POINT TYROSINASE OF AGARICUS-BISPORUS MAY BE A GLYCOPROTEIN [J].