A radioimmunoassay for serum estradiol

A radioimmunoassay procedure to measure estradiol levels in human serum was developed using I-125 labeled estradiol and antiestradiol antibody raised in-house using the estradiol-6-CMO-BSA as the immunogen. Controlled process serum was used to maintain the serum matrix and sample-standard identity. The assay was validated by analysing several (n = 40) samples and comparing with established commercial assays. The assay had a sensitivity of 35 pg/mL and a range of 50 pg/mL to 5 ng/mL. The interassay C.V were between 2 to 10% while the intraassay C.V were between 2 and 8%. This assay is sensitive enough for monitoring ovulation and hypersecretions in ovarian rumours and was primarily developed to be used as an in-house assay in the local hospitals.