Aberrant methylation patterns at the two-cell stage as an indicator of early developmental failure

被引:188
作者
Shi, W
Haaf, T
机构
[1] Univ Mainz, Sch Med, Inst Human Genet, D-55131 Mainz, Germany
[2] Max Planck Inst Mol Genet, Berlin, Germany
关键词
embryo loss; epigenetic reprogramming; in vitro fertilization; methylcytosine immunofluorescence; preimplantation development; superovulation;
D O I
10.1002/mrd.90016
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The fertilized mouse egg actively demethylates the paternal genome within a few hours after fertilization, whereas the maternal genome is only passively demethylated by a replication-dependent mechanism after the two-cell stage. This evolutionarily conserved assymetry in the early diploid mammalian embryo may have a role in methylation reprogramming of the two very different sets of sperm and egg chromatin for somatic development and formation of totipotent cells. Immunofluorescence staining with an antibody against 5-methylcytosine (MeC) showed that the incidence of abnormal methylation patterns differs between mouse two-cell embryos from superovulated females, nonsuperovulated matings, and in vitro fertilization (IVF). It also depends on embryo culture conditions and genetic background. In general, there was a good correlation with the number of embryos (from the same experiment) which did not develop in vitro up to the blastocyst stage. Thus, aberrant genome-wide DNA methylation in early embryos may be an important mechanism contributing to the high incidence of developmental failure in mammals. Similar to the situation in abnormally methylated embryos from nuclear transfer, it may cause a high incidence of pregnancy loss and abnormal phenotypes.
引用
收藏
页码:329 / 334
页数:6
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