Screening for fractions of Oxytropis falcata Bunge with antibacterial activity

被引:55
作者
Jiang, H. [1 ]
Hu, J. R. [1 ]
Zhan, W. Q. [1 ]
Liu, X. [2 ]
机构
[1] Gansu Acad Tradit Chinese Med, Lanzhou 730050, Peoples R China
[2] Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China
关键词
Oxytropis falcate Bunge; extracts; flavonoid compounds; antibacterial activity; FLAVONOIDS; CHALCONES;
D O I
10.1080/14786410902906934
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Preliminary studies with the four extracts of Oxytropis falcate Bunge exhibited that the chloroform and ethyl acetate extracts showed stronger antibacterial activities against the nine tested Gram-positive and Gram-negative bacteria. The HPLC-scanned and bioassay-guided fractionation led to the isolation and identification of the main flavonoid compounds, i.e. rhamnocitrin, kaempferol, rhamnetin, 2',4'-dihydroxychalcone and 2',4',-trihydroxy-dihydrochalcon. Except 2',4',-trihydroxy-dihydrochalcon, four other compounds had good antibacterial activities. The minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) of the four compounds ranged between 125 and 515 mu g mL-1. Staphylococcus aureus was the most susceptible to these compounds, with MIC and MBC values from 125 to 130 mu g mL-1. This is the first report of antibacterial activity in O. falcate Bunge. In this study, evidence to evaluate the biological functions of O. falcate Bunge is provided, which promote the rational use of this herb.
引用
收藏
页码:953 / 959
页数:7
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