Determination of sulfonamides in selected Malaysian swine wastewater by high-performance liquid chromatography

被引：100

作者：

Malintan, Nancy T. ^{[1
]}

Mohd, Mustafa Ali ^{[1
]}

机构：

[1] Univ Malaya, Fac Med, Dept Pharmacol, Kuala Lumpur 50603, Malaysia

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1127卷 / 1-2期

关键词：

antibiotic; sulfonamide; HPLC; waste water; aquatic environment; pharmaceutical contaminant;

D O I：

10.1016/j.chroma.2006.06.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An analytical HPLC method for the simultaneous determination of eight sulfonamides in swine wastewater was developed. The samples were collected from three states in Malaysia. Sample clean up was carried out by employing solid-phase extraction using a 60 mg Oasis HLB (Waters) cartridge with 3 ml reservoir. The HPLC column used was Supelcosil C18 (250 mm x 4.6 mm I.D.) and elution was carried out using gradient mode. The mobile phases used were acetonitrile and 0.5% acetic acid in purified water. Antibiotics were detected using UV absorbance at 272 nm. Recoveries obtained for sulphanilamide ranged from 31.9 +/- 5.1% to 36.2 +/- 1.0%, while recoveries for other sulfa drugs studied were from 91.9 +/- 5.0% to 106.0 +/- 1.1%. The limit of quantitation (LOQ) for sulfamerazine, sulfamethazine and sulfamethoxypyridazine was 7.5 ng/L, while the LOQ for the other studied antibiotics was 5.0 ng/L. The method was used to analyse sulfonamides in wastewater collected from selected Malaysian swine facilities. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：154 / 160

页数：7

共 23 条

[1]

Alder A.C., 2001, PHARM PERSONAL CARE, VVolume 791, P56, DOI [10.1021/bk-2001-0791.ch003, DOI 10.1021/BK-2001-0791.CH003, 10.1021/bk-2001-0791, DOI 10.1021/BK-2001-0791]

[2] Occurrence of sulfonamide antimicrobials in private water wells in Washington County, Idaho, USA [J].

Batt, Angela L. ;

Snow, Daniel D. ;

Aga, Diana S. .

CHEMOSPHERE, 2006, 64 (11) :1963-1971

[3] Antimicrobial residues in animal waste and water resources proximal to large-scale swine and poultry feeding operations [J].

Campagnolo, ER ;

Johnson, KR ;

Karpati, A ;

Rubin, CS ;

Kolpin, DW ;

Meyer, MT ;

Esteban, JE ;

Currier, RW ;

Smith, K ;

Thu, KM ;

McGeehin, M .

SCIENCE OF THE TOTAL ENVIRONMENT, 2002, 299 (1-3) :89-95

[4] Quantitative determination of sulfonamide in meat by liquid chromatography-electrospray-mass spectrometry [J].

Fuh, MRS ;

Chan, SA .

TALANTA, 2001, 55 (06) :1127-1139

[5] METHODS FOR THE DETERMINATION OF SULFONAMIDES IN MEAT [J].

GUGGISBERG, D ;

MOOSER, AE ;

KOCH, H .

JOURNAL OF CHROMATOGRAPHY, 1992, 624 (1-2) :425-437

[6] Quantification of veterinary antibiotics (sulfonamides and trimethoprim) in animal manure by liquid chromatography-mass spectrometry [J].

Haller, MY ;

Müller, SR ;

McArdell, CS ;

Alder, AC ;

Suter, MJF .

JOURNAL OF CHROMATOGRAPHY A, 2002, 952 (1-2) :111-120

[7] Detection and identification of sulphonamide drugs in municipal waste water by liquid chromatography coupled with electrospray ionisation tandem mass spectrometry [J].

Hartig, C ;

Storm, T ;

Jekel, M .

JOURNAL OF CHROMATOGRAPHY A, 1999, 854 (1-2) :163-173

[8] Tracking persistent pharmaceutical residues from municipal sewage to drinking water [J].

Heberer, T .

JOURNAL OF HYDROLOGY, 2002, 266 (3-4) :175-189

[9] Occurrence, fate, and removal of pharmaceutical residues in the aquatic environment: a review of recent research data [J].

Heberer, T .

TOXICOLOGY LETTERS, 2002, 131 (1-2) :5-17

[10] Determination of sulfonamides in animal tissues using cation exchange reversed phase sorbent for sample cleanup and HPLC-DAD for detection [J].

Hela, W ;

Brandtner, M ;

Widek, R ;

Schuh, R .

FOOD CHEMISTRY, 2003, 83 (04) :601-608

← 1 2 3 →