Oxidative stress during aging of stationary cultures of the yeast Saccharomyces cerevisiae

被引：151

作者：

Jakubowski, W

Bilinski, T

Bartosz, G

机构：

[1] Univ Lodz, Dept Mol Biophys, PL-90137 Lodz, Poland

[2] Pedagog Univ Rzeszow, Inst Biol & Environm Sci, Rzeszow, Poland

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 2000年 / 28卷 / 05期

关键词：

yeast; Saccharomyces cerevisiae; aging; oxidative stress; stationary culture; superoxide dismutase; catalase; glutathione; protein carbonyl; free radicals;

D O I：

10.1016/S0891-5849(99)00266-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Comparison of 5 d old stationary cultures of Saccharomyces cerevisiae and of cultures aged for 3 months revealed increased generation of reactive oxygen species assessed by 2',7'-dichlorofluorescin oxidation, decreased activity of superoxide dismutase, decreased content of glutathione and increased protein carbonyl content during prolonged incubation of stationary yeast cultures. These results point to the occurrence of oxidative stress during aging of stationary cultures of the yeast. The magnitude of this stress was augmented in antioxidant-deficient strains, devoid of superoxide dismutases and catalases, and of decreased glutathione content. (C) 2000 Elsevier Science Inc.

引用

页码：659 / 664

页数：6

共 32 条

[1]

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[2] Passage through stationary phase advances replicative aging in Saccharomyces cerevisiae [J].