Effects of 3-D clino-rotation on gene expression in human fibroblast cells

被引：16

作者：

Arase, Y ^{[1
]}

Nomura, J ^{[1
]}

Sugaya, S ^{[1
]}

Sugita, K ^{[1
]}

Kita, K ^{[1
]}

Suzuki, N ^{[1
]}

机构：

[1] Chiba Univ, Grad Sch Med, Dept Environm Biochem, Chiba 2608670, Japan

来源：

CELL BIOLOGY INTERNATIONAL | 2002年 / 26卷 / 03期

关键词：

clino-rotation; 3-D clinostat; cDNA array; human fibroblast cells;

D O I：

10.1006/cbir.2001.0833

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 [细胞生物学]; 090102 [作物遗传育种];

摘要：

Continuous variation in the direction of the gravity vector leads to various cellular responses including modulation of gene expression. Complementary DNA (cDNA) array analyses are available to observe the variation of gene expression under different conditions. In this study, expression levels of 588 representative genes were compared using the Atlas(TM) human cDNA expression array in human fibroblast cells with and without 3-dimensional (3-D) clinostat. Five upregulated and 8 downregulated genes were detected. Among these genes, upregulation of XRCC1, and downregulation of ERB-B2 and p21(Cip/Waf1) were confirmed by RT-PCR. These results suggested that the gene expression levels of XRCC1, ERB-B2 and p21(Cip1/Waf1) were modulated by vector-averaged microgravity induced by 3-D clinostat in human fibroblast cells. Our findings may be a basis for the biological study of 3-D culture systems. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：225 / 233

页数：9

共 64 条

[1]

Cardiac organogenesis in vitro:: Reestablishment of three-dimensional tissue architecture by dissociated neonatal rat ventricular cells [J].

Akins, RE ;

Boyce, RA ;

Madonna, ML ;

Schroedl, NA ;

Gonda, SR ;

McLaughlin, TA ;

Hartzell, CR .

TISSUE ENGINEERING, 1999, 5 (02) :103-118

[2]

An improved quantitative RT-PCR fluorescent method for analysis of gene transcripts in the STS-65 space shuttle experiment [J].

Akiyama, H ;

Kanai, S ;

Hirano, M ;

Kumei, Y ;

Shimokawa, H ;

Katano, H ;

Hara, E ;

Song, SL ;

Mukai, C ;

Nagaoka, S .

JOURNAL OF BIOTECHNOLOGY, 1996, 47 (2-3) :325-333

[3]

Backert S, 1999, INT J CANCER, V82, P868, DOI 10.1002/(SICI)1097-0215(19990909)82:6<868::AID-IJC16>3.0.CO

[4]

2-W

[5]

Baker TL, 1997, IN VITRO CELL DEV-AN, V33, P358

[6]

Begley CM, 2000, BIOTECHNOL BIOENG, V70, P32, DOI 10.1002/1097-0290(20001005)70:1<32::AID-BIT5>3.0.CO

[7]

2-V

[8]

Growth factor-induced signal transduction in adherent mammalian cells is sensitive to gravity [J].

Boonstra, J .

FASEB JOURNAL, 1999, 13 :S35-S42

[9]

Botchwey EA, 2001, J BIOMED MATER RES, V55, P242

[10]

EMBRYOGENESIS AND ORGANOGENESIS OF CARAUSIUS-MOROSUS UNDER SPACEFLIGHT CONDITIONS [J].

BUCKER, H ;

HORNECK, G ;

REITZ, G ;

GRAUL, EH ;

BERGER, H ;

HOFFKEN, H ;

RUTHER, W ;

HEINRICH, W ;

BEAUJEAN, R .

NATURWISSENSCHAFTEN, 1986, 73 (07) :433-434

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