Cloning of a novel member (ARL5) of the ARF-family of ras-related GTPases

被引：15

作者：

Breiner, M ^{[1
]}

Schurmann, A ^{[1
]}

Becker, W ^{[1
]}

Joost, HG ^{[1
]}

机构：

[1] RHEIN WESTFAL TH AACHEN,INST PHARMAKOL & TOXIKOL,D-52057 AACHEN,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1996年 / 1308卷 / 01期

关键词：

ADP-ribosylation factor; GTPase; ras-homologous; ADP-RIBOSYLATION FACTOR; GTP-BINDING PROTEINS; PHOSPHOLIPASE-D; SACCHAROMYCES-CEREVISIAE; DROSOPHILA-MELANOGASTER; CHOLERA-TOXIN; GENES; EXPRESSION; MEMBRANES; TRANSPORT;

D O I：

10.1016/0167-4781(96)00081-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel ras-related GTPase with a unique structure was cloned by PCR-amplification with degenerate primers and screening of a rat fat cell cDNA library. The deduced amino acid sequence of the cDNA comprises all 6 GTP binding motifs which are conserved in Ras-related GTPases. The sequence is similar to that of ADP-ribosylation factors (ARF), and shows several structural features typical for the ARF-family. Because its closest relative is the GTPase ARL1 (49% identical amino acids, 54% identical nucleotides within the coding region), the protein was designated ARL5 (ARF-like protein 5). Low amounts of mRNA were found in most rat tissues examined (heart, skeletal muscle, fat, liver, kidney, lung, spleen, intestine, testis, and thymus) with highest levels in brain, intestine, and thymus.

引用

页码：1 / 6

页数：6

共 30 条

[1] MOLECULAR-CLONING, CHARACTERIZATION, AND EXPRESSION OF HUMAN ADP-RIBOSYLATION FACTORS - 2 GUANINE NUCLEOTIDE-DEPENDENT ACTIVATORS OF CHOLERA-TOXIN [J].

BOBAK, DA ;

NIGHTINGALE, MS ;

MURTAGH, JJ ;

PRICE, SR ;

MOSS, J ;

VAUGHAN, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (16) :6101-6105

[2] PROTEINS REGULATING RAS AND ITS RELATIVES [J].

BOGUSKI, MS ;

MCCORMICK, F .

NATURE, 1993, 366 (6456) :643-654

[3]

BOURNE HR, 1991, NATURE, V349, P117, DOI 10.1038/349117a0

[4] ADP-RIBOSYLATION FACTOR, A SMALL GTP-DEPENDENT REGULATORY PROTEIN, STIMULATES PHOSPHOLIPASE-D ACTIVITY [J].

BROWN, HA ;

GUTOWSKI, S ;

MOOMAW, CR ;

SLAUGHTER, C ;

STERNWEIS, PC .

CELL, 1993, 75 (06) :1137-1144

[5]

CAVENAGH MM, 1994, J BIOL CHEM, V269, P18937

[6] SELECTIVE AMPLIFICATION OF ADDITIONAL MEMBERS OF THE ADP-RIBOSYLATION FACTOR (ARF) FAMILY - CLONING OF ADDITIONAL HUMAN AND DROSOPHILA ARF-LIKE GENES [J].

CLARK, J ;

MOORE, L ;

KRASINSKAS, A ;

WAY, J ;

BATTEY, J ;

TAMKUN, J ;

KAHN, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (19) :8952-8956

[7] PHOSPHOLIPASE-D - A DOWNSTREAM EFFECTOR OF ARF IN GRANULOCYTES [J].

COCKCROFT, S ;

THOMAS, GMH ;

FENSOME, A ;

GENY, B ;

CUNNINGHAM, E ;

GOUT, I ;

HILES, I ;

TOTTY, NF ;

TRUONG, Q ;

HSUAN, JJ .

SCIENCE, 1994, 263 (5146) :523-526

[8] A REGULATORY ROLE FOR ARF6 IN RECEPTOR-MEDIATED ENDOCYTOSIS [J].

D'SOUZA-SCHOREY, C ;

LI, GP ;

COLOMBO, MI ;

STAHL, PD .

SCIENCE, 1995, 267 (5201) :1175-1178

[9] BINDING OF ARF AND BETA-COP TO GOLGI MEMBRANES - POSSIBLE REGULATION BY A TRIMERIC G-PROTEIN [J].

DONALDSON, JG ;

KAHN, RA ;

LIPPINCOTTSCHWARTZ, J ;

KLAUSNER, RD .

SCIENCE, 1991, 254 (5035) :1197-1199

[10] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

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