Mandibular reconstruction in miniature pigs with prefabricated vascularized bone grafts using recombinant human osteogenic protein-1: a preliminary study

被引:36
作者
Terheyden, H
Jepsen, S
Rueger, DR
机构
[1] Univ Kiel, Dept Oral & Maxillofacial Surg, D-24105 Kiel, Germany
[2] Univ Kiel, Dept Restorat Dent & Peridontol, D-24098 Kiel, Germany
关键词
bone morphogenetic proteins; mandibular reconstruction; surgical flaps;
D O I
10.1034/j.1399-0020.1999.280613.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
A new technique of prefabrication of vascularized bone grafts in the latissimus dorsi muscle of miniature pigs, using recombinant human osteogenic protein-1 (rhOP-1) and xenogenic bone mineral as a carrier, is presented. The grafts were used to reconstruct a mandible using microsurgical anastomosis of the thoracodorsal vessels of the flap with a branch of the external carotid artery and the external jugular vein. The technique provided enough bone to reconstruct a mandible. The bone marrow stayed viable after transplantation, demonstrated by fluorescence microscopy and scintigraphy. Compared with identical defects of the contralateral side, which were treated with directly applied rhOP-1/bone mineral, a significantly better result was obtained with the prefabrication technique. The prefabrication technique may provide a means to treat irradiated patients with bone grafts produced by osteoinductive proteins and has a potential to become a clinical alternative to conventional vascularized bone grafts.
引用
收藏
页码:461 / 463
页数:3
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