Two-photon excitation of ethidium bromide labeled DNA

被引：29

作者：

Malak, H ^{[1
]}

Castellano, FN ^{[1
]}

Gryczynski, I ^{[1
]}

Lakowicz, JR ^{[1
]}

机构：

[1] UNIV MARYLAND, SCH MED, DEPT BIOCHEM & MOL BIOL, CTR FLUORESCENCE SPECT, BALTIMORE, MD 21201 USA

来源：

BIOPHYSICAL CHEMISTRY | 1997年 / 67卷 / 1-3期

关键词：

fluorescence spectroscopy; fluorescence microscopy; imaging; time-resolved fluorescence; two-photon excitation; three-photon excitation; DNA; ethidium bromide; chromosomes; multi-photon microscopy;

D O I：

10.1016/S0301-4622(97)00017-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined the steady state and time-resolved emission of DNA stained with ethidium bromide (EB) when excited with 90 fs pulses from a mode-locked titanium sapphire laser. Over the wavelength range from 840 to 880 nm EB-DNA was found to display two-photon excitation, with a cross-section near 7 X 10(-50) cm(4)s/photon. Frequency-domain intensity decay measurements revealed similar multi-exponential intensity decays for one-and two-photon excitation, Time-resolved anisotropy decay measurements revealed similar correlation times, but different amplitudes as has been observed previously for two-versus one-photon excitation. These results indicate that two-photon excitation of EB-DNA can be accomplished with the fundamental output of a Ti:sapphire laser without obvious heating or perturbation of the DNA. (C) 1997 Elsevier Science B.V.

引用

页码：35 / 41

页数：7

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