Two Chromatin Remodeling Activities Cooperate during Activation of Hormone Responsive Promoters

被引：42

作者：

Pablo Vicent, Guillermo ^{[1
]}

Zaurin, Roser ^{[1
]}

Silvina Nacht, A. ^{[1
]}

Li, Ang ^{[1
]}

Font-Mateu, Jofre ^{[1
]}

Le Dily, Francois ^{[1
]}

Vermeulen, Michiel ^{[2
,3
,4
]}

Mann, Matthias ^{[2
]}

Beato, Miguel ^{[1
]}

机构：

[1] Univ Pompeu Fabra, CRG, Barcelona, Spain

[2] Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany

[3] Univ Med Ctr Utrecht, Dept Physiol Chem, Utrecht, Netherlands

[4] Univ Med Ctr Utrecht, Canc Genom Ctr, Utrecht, Netherlands

来源：

PLOS GENETICS | 2009年 / 5卷 / 07期

关键词：

TUMOR VIRUS PROMOTER; HISTONE ACETYLTRANSFERASE COMPLEXES; MMTV PROMOTER; PROGESTERONE-RECEPTOR; IN-VIVO; TRANSCRIPTIONAL ACTIVATION; GLUCOCORTICOID-RECEPTOR; H1; PHOSPHORYLATION; ESTROGEN-RECEPTOR; DIVERSE FUNCTIONS;

D O I：

10.1371/journal.pgen.1000567

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Steroid hormones regulate gene expression by interaction of their receptors with hormone responsive elements (HREs) and recruitment of kinases, chromatin remodeling complexes, and coregulators to their target promoters. Here we show that in breast cancer cells the BAF, but not the closely related PBAF complex, is required for progesterone induction of several target genes including MMTV, where it catalyzes localized displacement of histones H2A and H2B and subsequent NF1 binding. PCAF is also needed for induction of progesterone target genes and acetylates histone H3 at K14, an epigenetic mark that interacts with the BAF subunits by anchoring the complex to chromatin. In the absence of PCAF, full loading of target promoters with hormone receptors and BAF is precluded, and induction is compromised. Thus, activation of hormone-responsive promoters requires cooperation of at least two chromatin remodeling activities, BAF and PCAF.

引用

页数：13

共 72 条

[1] What does 'chromatin remodeling' mean? [J].