Purification, characterization, and cDNA cloning of a new fibrinogenlytic venom protein, Agkisacutacin, from Agkistrodon acutus venom

被引：21

作者：

Cheng, X ^{[1
]}

Qian, YW ^{[1
]}

Liu, QD ^{[1
]}

Li, BXY ^{[1
]}

Zhang, MW ^{[1
]}

Liu, J ^{[1
]}

机构：

[1] Univ Sci & Technol China, Dept Biochem & Mol Biol, Sch Life Sci, Hefei 230027, Anhui, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 265卷 / 02期

关键词：

D O I：

10.1006/bbrc.1999.1685

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Agkisacutacin is a new fibrinogenlytic protein from Agkistrodon acutus venom. It consists of two heterologous subunits linked by an intersubunit disulfide bond, The cDNAs encoding the two chains of Agkisacutacin were cloned from a lambda gt11 cDNA library of the snake venom gland and sequenced, including the leader peptides (23/23 amino acid residues) and mature subunits (129/123 amino acid residues). It is structurally related to the family of IX/X-binding protein (IX/X-bp)-like proteins and shows high similarity (alpha-70%/beta-64%) to habu IX/X-bp from Trimeresurus flavoridis, but displays distinct biological activity with direct action on fibrinogen, (C) 1999 Academic Press.

引用

页码：530 / 535

页数：6

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