PTX3 plays a key role in the organization of the cumulus oophorus extracellular matrix and in in vivo fertilization

被引:368
作者
Salustri, A
Garlanda, C
Hirsch, E
De Acetis, M
Maccagno, A
Bottazzi, B
Doni, A
Bastone, A
Mantovani, G
Peccoz, PB
Salvatori, G
Mahoney, DJ
Day, AJ
Siracusa, G
Romani, L
Mantovani, A [1 ]
机构
[1] Univ Roma Tor Vergata, Dept Publ Hlth & Cell Biol, I-00133 Rome, Italy
[2] Mario Negri Inst Pharmacol Res, Dept Immunol & Cell Biol, I-20157 Milan, Italy
[3] Univ Turin, Dept Genet Biol & Biochem, I-10126 Turin, Italy
[4] Univ Milan, Inst Endocrinol, I-20100 Milan, Italy
[5] SigmaTau SpA, I-00040 Monte Porzio Catone, Italy
[6] Univ Oxford, MRC, Immunochem Unit, Dept Biochem, Oxford OX1 3QU, England
[7] Univ Perugia, Dept Expt Med & Biochem Sci, Microbiol Sect, I-05122 Perugia, Italy
[8] Univ Milan, Inst Gen Pathol, Ctr IDET, I-20100 Milan, Italy
来源
DEVELOPMENT | 2004年 / 131卷 / 07期
关键词
cumulus oophorus; extracellular matrix; fertility; pentraxins; mouse;
D O I
10.1242/dev.01056
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
PTX3 is a prototypic long pentraxin that plays a non-redundant role in innate immunity against selected pathogens and in female fertility. Here, we report that the infertility of Ptx3(-/-) mice is associated with severe abnormalities of the cumulus oophorus and failure of in vivo, but not in vitro, oocyte fertilization. PTX3 is produced by mouse cumulus cells during cumulus expansion and localizes in the matrix. PTX3 is expressed in the human cumulus oophorus as well. Cumuli from Ptx3(-/-) mice synthesize normal amounts of hyaluronan (HA), but are unable to organize it in a stable matrix. Exogenous PTX3 restores a normal cumulus phenotype. Incorporation in the matrix of inter-a-trypsin inhibitor is normal in Ptx3(-/-) cumuli. PTX3 does not interact directly with HA, but it binds the cumulus matrix hyaladherin tumor necrosis factor a-induced protein 6 (TNFAIP6, also known as TSG6) and thereby may form multimolecular complexes that can cross-link HA chains. Thus, PTX3 is a structural constituent of the cumulus oophorus extracellular matrix essential for female fertility.
引用
收藏
页码:1577 / 1586
页数:10
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